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目的观察孕妇外周血和新生儿脐带血内皮祖细胞(endothelial progenitor cells,EPCs)数量和功能变化的相关性。方法用流式细胞仪检测未怀孕妇女和早孕妇女外周血中内皮祖细胞的数量;用密度梯度离心法分离脐带血内皮祖细胞;用流式细胞仪检测脐带血内皮祖细胞的数量;脐带血内皮祖细胞经体外培养7d后,分组做增殖实验、迁移实验和粘附实验。结果妇女怀孕后循环中内皮祖细胞数量增加;外周血内皮祖细胞数量明显降低的孕妇所产新生儿的脐带血内皮祖细胞数量降低,增殖、迁移和粘附能力下降,与对照组比较差异有统计学意义(P<0.05)。结论怀孕后循环内皮祖细胞数量增加明显;孕妇外周血和新生儿脐带血内皮祖细胞的数量和功能变化有一定的相关性。
Objective To observe the correlation between the quantity and function of endothelial progenitor cells (EPCs) in peripheral blood of pregnant women and neonates. Methods Flow cytometry was used to detect the number of endothelial progenitor cells in peripheral blood of non-pregnant women and pregnant women. Umbilical cord blood endothelial progenitor cells were separated by density gradient centrifugation. The number of cord blood progenitor cells was detected by flow cytometry. After cultured in vitro for 7 days, EPCs were proliferated, migrated and adhered. Results The number of circulating endothelial progenitor cells increased after pregnancy in pregnant women. The number of neonatal umbilical cord blood endothelial progenitor cells produced by pregnant women with a significantly decreased number of peripheral endothelial progenitor cells decreased, the proliferation, migration and adhesion decreased, compared with the control group Statistical significance (P <0.05). Conclusions The number of circulating endothelial progenitor cells increased significantly after pregnancy. There was a correlation between the number of cord blood progenitor cells in peripheral blood of pregnant women and neonatal umbilical cord blood and functional changes.