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目的 探讨干细胞更新因子Bmi-1、干细胞表面标志物巢蛋白Nestin在皮肤黑素瘤中表达的临床与病理意义.方法 收集武汉市妇女儿童医疗保健中心、荆州市中心医院2010-2015年40例经手术切除经组织病理加免疫组化诊断为黑素瘤的石蜡包埋切片为研究对象(实验组),选择40例组织病理诊断为良性色素痣标本作为阴性对照(对照组).SP免疫组化法分别检测实验组与对照组Bmi-1、Nes-tin的表达与患者性别、年龄、发病部位、肿块大小、病理分级、临床分期等因素是否具有差异;并对Bmi-1及Nestin行相关性统计分析.结果 黑素瘤组中Bmi-1、Nestin高表达,且Bmi-1、Nestin的高表达与肿瘤大小、病理分级、临床分期密切相关;Bmi-1与Nestin的表达呈正相关.结论 Bmi-1和Nestin在肿瘤的发生、发展中可能存在相关性,可能是一个新的皮肤黑素瘤辅助诊断组织学标记.“,”Objective To explore the clinical and pathological significance of both Bmi-1 and Nestin in the expression of malignant melanoma.Methods Tumor samples were collected from 40 cases melanoma patients and 40 cases melanotic nevus patients between 2010-2015 in Wuhan Medical and Health Center for Women and Children,Jingzhou Central Hospital.All specimens by confirmed by pathologic examination with S-100 and HMB45 immunohistochemistry.The expression of Bmi-1 and Nestin was detected with SP immunohistochemical method in 40 cases of melanoma and 40 cases of melanotic nevus.The difference analysis of the expression of Bmi-1/nestin and sex,age,tumor site,tumor size,athologic classification,clinical stage,lymph node and distant metastasis were studied by SPSS software respectively.Furthermore,the correlation analysis between Bmi-1 and nestin was also detected by SPSS software.Results The increased Bmi-1 and nestin expression in melanoma indicates that Bmi-1 and Nestin might be associated with the progression of melanoma;The high expression of Nestin and Bmi-1 was closely related to tumor size,lymphnode metastasis,pathological grading and clinical stage;The significant positive correlation between Bmi-1 and Nestin expression indicates that Bmi-1 and Nestin might be related with development of malignant melanoma.Conclusion Bmi-1 and nestin may be new histological markers for the diagnosis of the melanoma.