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在从摩洛哥引进的脐血橙田间植株的新梢嫩叶上观察到褪绿环斑和污斑,褪绿斑驳,叶背有边缘凹刻的圆形或近圆形环,以及脉明和叶脉环阻等症状。通过向MadamVinous甜橙、邓肯葡萄柚等柑桔类指示植物的嫁接接种;用钝刀醮取汁液切割指示植物墨西哥来檬和埃特洛香的茎部进行机械传播;以及汁液磨擦接种草本指示植物昆诺藜等方法,鉴定到柑桔环斑病毒(citrusringspotvirus,CRSV)。嫁接接种的柑桔类指示植物上的症状包括新梢休克、新叶脱落,新叶表现褪绿环斑和污斑以及斑驳,脉明和叶脉环阻等;机械接种的柑桔类指示植物表现新叶的褪绿环斑症状;昆诺藜在接种后4~6d于接种叶上产生褪绿至坏死的局部枯斑,但无系统感染的症状出现。取表现症状的柑桔类指示植物和昆诺藜的叶片作接种源,汁液磨擦接种回接至昆诺藜,再现了同样的症状。
Chloroplast spot and stain, chlorotic mottled, circular or nearly circular ring with concave edge on the leaf margin, and vein and vein rings were observed on the tender shoots of the shoots of cord blood orange plants introduced from Morocco Resistance and other symptoms. Grafting was inoculated by indicating plants to citrus such as MadamVinous orange and Duncan grapefruit; cutting the stems of the indica plants Mexican camellia and Etroplon with a blunt cuticle for mechanical transmission; and juice grazing of herbaceous indica plants Quinoa and other methods, identified to citrus ring spot virus (citrusringspotvirus, CRSV). Grafted inoculated citrus indicates symptoms on plants including shoot shock, shedding of new leaves, chlorotic and spotted chloroplasts in new leaves, mottling and vein resistance, etc .; mechanically inoculated citrus plants indicating new Leaf chlorotic symptoms; Quinoa 4 to 6d after inoculation inoculation leaves produce chlorotic to necrotic local spot, but no symptoms of systemic infection. Take the symptoms of citrus indica plants and leaves of quinoa as a source of inoculation, the juice friction back to the quinoa to reproduce the same symptoms.