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哺乳动物精子在培养液中可诱发自发的顶体反应,与透明带结合后可引起透明带诱导的顶体反应。在人所知尚少,本研究用盐贮存人卵的透明带,探讨透明带诱导的顶体反应,以及宫颈粘液(CM)对其影响。手淫法采精,室温下30分钟液化后,45%Percoll 2.0ml、900×g离心20分钟,所得精子沉积,加mBWW混匀,300×g离心10分钟,去上清液,制成0.5ml高浓度精子悬液,加2.0ml mBWW用上游法选活动良好的精子。用1ml结核菌素注射器分别吸取0.2ml mBWW和0.2ml成年妇女排卵日的CM,二者不混合呈分层状态,CM层下方垂
Mammalian spermatozoa can induce a spontaneous acrosome reaction in the culture fluid, which in combination with the zona pellucida can cause the zona pellucida-induced acrosome reaction. To the best of my knowledge, this study used zona pellucida stored in zona pellucida to investigate zona pellucida-induced acrosome reaction and the effects of cervical mucus (CM) on it. Masturbation method ejaculation, after liquefaction for 30 minutes at room temperature, 45% Percoll 2.0ml, centrifuged at 900 × g for 20 minutes, the resulting sperm deposited, add mBWW mix, centrifuge at 300 × g for 10 minutes to the supernatant to make 0.5ml High concentrations of sperm suspension, add 2.0ml mBWW using the upstream method to select good sperm activity. With 1ml tuberculin syringe were drawn 0.2ml mBWW and 0.2ml adult women Ovulation day CM, the two were not mixed layered state, CM layer down