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一、试管苗的培养(一)培养方法可分为固体培养和液体培养两大类.1.固体培养在培养基中加入一定量的凝固剂配制而成,常用的凝固剂有琼脂,使用浓度为0.6-1%(重量/体积).固体培养的优点是实验设备简单,操作技术容易掌握,便于在生产上推广.缺点是外植体材料只有一部分与培养基接触,而且培养一段时间后,因外植体周围的营养被吸收后造成培养基营养物质分布不均匀和有毒代谢物质的积累.
First, the test tube seedling culture (A) culture methods can be divided into solid culture and liquid culture two categories .1 solid culture in the medium by adding a certain amount of coagulant formulated, commonly used coagulant agar, the use of concentration Is 0.6-1% (weight / volume) solid culture has the advantage of simple experimental equipment, easy to grasp the operating techniques, easy to promote in the production.The disadvantage is that only part of the explant material with the media contact, and after a period of culture, Due to the explants around the nutrition is absorbed, resulting in uneven distribution of nutrients in the medium and the accumulation of toxic metabolites.