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采用乙酰胆碱酯酶（ＡｃｈＥ）单抗结合荧光免疫标记红细胞，通过流式细胞仪直接对单个红细胞膜上ＡｃｈＥ进行定量检测，发现阵发性睡眠性血红蛋白尿症（ＰＮＨ）患者体内红细胞可分为两个类群，一群ＡｃｈＥ含量正常（ＡｃｈＥ（＋）］，一群ＡｃｈＥ缺失［ＡｃｈＥ（一）］；ＡｃｈＥ（－）红细胞百分率与蛇毒因子（ＣｏＦ）诱发的补体溶血率呈正相关；经ＣｏＦ诱发补体溶血后，仍可发现有对补体不敏感但无ＡｃｈＥ的异常红细胞，说明对补体不敏感的红细胞仍有其它异常。本法有可能成为一种比补体溶血试验更灵敏、更特异并易定量的ＰＮＨ诊断方法。
AchE monoclonal antibody and fluorescent immunofluorescent labeled erythrocytes were used to quantitatively detect AchE on a single erythrocyte membrane directly by flow cytometry and found that erythrocytes in patients with paroxysmal nocturnal hemoglobinuria (PNH) can be divided into two A group of AchE (+)], a group of AchE deletion (AchE); the percentage of AchE (-) erythrocytes was positively correlated with the rate of complement hemolysis induced by snake venom factor (CoF) , There are still abnormal red blood cells that are not sensitive to complement but not AchE, indicating that there are other abnormalities in erythrocytes that are not sensitive to complement.This method may become a more sensitive, specific and quantitative PNH diagnosis than complement hemolysis method.