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目的观察替吉奥联合草酸铂对白细胞介素-6(IL-6)诱导的胃癌细胞侵袭的影响,并探究其机制。方法选取人胃癌SGC7901细胞,设对照组、IL-6组、IL-6+替吉奥组、IL-6+草酸铂组和IL-6+替吉奥+草酸铂组;IL-6组滴入50 ng/ml IL-6,IL-6+替吉奥组滴入50 ng/ml+替吉奥20 mg/L,IL-6+草酸铂组滴入50 ng/ml IL-6+20 mg/L草酸铂,IL-6+替吉奥+草酸铂组滴入50 ng/ml IL-6+20 mg/L替吉奥+20 mg/L草酸铂,对照组未接受任何药物干预。用MTT方法测定其对细胞生长抑制作用,Transwell法测定细胞侵袭能力,ELISA法测定细胞上清液中MMP-2、VEGF水平,Western blotting方法检测细胞中AKT、p-AKT蛋白的表达水平,并对结果进行比较。结果在24、48和72 h时IL-6+替吉奥+草酸铂组胃癌细胞的生长抑制率明显的高于IL-6+替吉奥组和IL-6+草酸铂组,差异具有统计学意义(P<0.01);替吉奥+草酸铂对IL-6作用的胃癌细胞侵袭率明显的高于单一的替吉奥或草酸铂,差异具有统计学意义(P<0.01);IL-6+替吉奥+草酸铂组上层细胞液中的MMP-2、VEGF水平明显的低于IL-6+替吉奥组和IL-6+草酸铂组;IL-6+替吉奥+草酸铂组中AKT的表达量明显高于IL-6+替吉奥组和IL-6+草酸铂组,p-AKT的表达量明显的低于IL-6+替吉奥组和IL-6+草酸铂组,差异有统计学意义(P<0.05)。结论替吉奥联合草酸铂可以抑制胃癌SGC7901细胞侵袭,其机制可能与调控ATK信号通路有关。
Objective To observe the effects of tioguanoside combined with oxaliplatin on the invasion of gastric cancer cells induced by interleukin-6 (IL-6) and to explore its mechanism. Methods Human gastric cancer SGC7901 cells were divided into four groups: control group, IL-6 group, IL-6 + tioglitazone group, IL-6 + oxaliplatin group and IL-6 + 50 ng / ml IL-6 + 20 μg / ml and 20 μg / ml TG-IL-6 + / L oxaliplatin and IL-6 + Tegmaol + oxaliplatin group, 50 ng / ml IL-6 + 20 mg / L tegaserod + 20 mg / L oxaliplatin was instilled. The control group did not receive any drug intervention. The cell growth inhibition was determined by MTT assay. The cell invasion was measured by Transwell method. The levels of MMP-2 and VEGF in the cell supernatant were measured by ELISA. The protein expression of AKT and p-AKT in cells was detected by Western blotting. Compare the results. Results The growth inhibition rate of gastric cancer cells in IL-6 + TIGO + oxaliplatin group at 24, 48 and 72 h was significantly higher than that of IL-6 + TIGO group and IL-6 + oxaliplatin group (P <0.01). The invasion rate of gastric cancer cells treated with tioglooxalate and oxaliplatin was significantly higher than that of single dose of tioguan or oxaliplatin (P <0.01) The levels of MMP-2 and VEGF in the supernatant of 6+ Tegtin + oxaliplatin group were significantly lower than that of IL-6 + TegGIO group and IL-6 + oxaliplatin group. IL-6 + The expression of AKT in platinum group was significantly higher than that in IL-6 + group and IL-6 + oxaliplatin group, the expression of p-AKT was significantly lower than that of IL-6 + Oxaliplatin group, the difference was statistically significant (P <0.05). Conclusion TIGO combined with oxaliplatin can inhibit the invasion of gastric cancer SGC7901 cells, which may be related to the regulation of ATK signaling pathway.