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目的：研究胃癌细胞耐药相关性单克隆抗体ＭＧｒ－１对耐长春新碱胃癌细胞（ＳＧＣ７９０１／ＶＣＲ）多药耐药性（ＭＤＲ）的影响。方法：用ＭＴＴ法检测阿霉素的细胞毒性；用流式细胞仪检测阿霉素在细胞内的蓄积及潴留。结果：ＭＧｒ－１能明显逆转ＳＧＣ７９０１／ＶＣＲ细胞对阿霉素的耐药性（Ｐ＜０．０１），并可增加ＳＧＣ７９０１／ＶＣＲ细胞内阿霉素的蓄积（Ｐ＜０．０１）及潴留（Ｐ＜０．０１）。结论：ＭＧｒ－１具有通过增加细胞内药物蓄积及潴留逆转ＳＧＣ７９０１／ＶＣＲ细胞耐药性的功能。提示ＭＧｒ－１所对应的细胞膜抗原可能为一种新的有活性功能并与ＭＤＲ相关的分子。
OBJECTIVE: To investigate the effect of multidrug resistance-related monoclonal antibody MGr-1 on gastric cancer cell line SGC7901/VCR resistant to multidrug resistance (MDR). Methods: The cytotoxicity of adriamycin was detected by MTT assay; the accumulation and retention of doxorubicin in cells were detected by flow cytometry. RESULTS: MGr-1 significantly reversed the doxorubicin resistance of SGC7901/VCR cells (P<0.01), and increased the accumulation of adriamycin in SGC7901/VCR cells (P<0.01) and retention. (P<0.01). CONCLUSION: MGr-1 has the function of reversing the drug resistance of SGC7901/VCR cells by increasing intracellular drug accumulation and retention. It suggests that the cell membrane antigen corresponding to MGr-1 may be a new molecule with active function and associated with MDR.