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目的观察健脾活血袪湿方对肝硬化腹水大鼠肝功能及肝组织水通道蛋白9(AQP 9)表达的影响。方法将Wistar大鼠,随机分为6组,即正常组,模型组,健脾活血祛湿方高、中、低剂量组(52,26,16.25 g·kg-1),秋水仙碱组(100滋g·kg-1),观察大鼠的一般情况,测定腹水量、检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)的含量及AQP 9 mRNA转录与蛋白翻译水平。结果健脾活血祛湿方对肝硬化腹水大鼠症状有明显的改善作用,能促进模型大鼠腹水消退、改善相应肝功能指标,具有良好的保肝作用;与模型组比较,健脾活血祛湿方组AQP 9 mRNA转录与蛋白翻译水平均有不同程度的升高,作用强度与用药剂量呈正相关。结论健脾活血祛湿方良好的保肝作用及对AQP 9的升高作用是其治疗肝硬化腹水的靶点之一。
Objective To observe the effects of Jianpi Huoxue Huo Dan Formula on the liver function and the expression of aquaporin 9 (AQP 9) in hepatic cirrhotic rats. Methods Wistar rats were randomly divided into 6 groups: normal group, model group, high, medium and low dose group (52,26,16.25 g · kg-1), colchicine group 100 μg · kg-1). The normal rats were observed. The amount of ascites was measured. The contents of ALT, AST, ALB and the level of AQP 9 mRNA transcript and protein translation . Results Jianpi Huoxue Qushi Decoction significantly improve the symptoms of cirrhotic rats, can promote ascites subsides, improve the corresponding liver function indicators, and has a good hepatoprotective effect; Compared with the model group, Wet prescription group AQP 9 mRNA transcription and protein translation levels were increased to varying degrees, the intensity of use and dose was positively correlated. Conclusion The good hepatoprotective effect of Jianpi Huoxue Qushi Decoction and its effect on AQP 9 are one of its targets in the treatment of ascites due to cirrhosis.