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研究冷晶体及温血停搏液对心肌的保护作用。方法：将９６只猫随机分为体外循环（ＣＰＢ）组（Ⅰ）、损伤组（Ⅱ）、冷晶保护组（Ⅲ）及温血保护组（Ⅳ）。观测各组心功能、膜ＡＴＰ酶活性和膜蛋白颗粒的变化。结果：组Ⅰ各时间点各项指标无变化。缺血期间，组Ⅱ～ⅣＮａ＋ ，Ｋ＋ －ＡＴＰａｓｅ 活性下降（Ｐ＜ ０．０１）；组Ⅱ，ⅢＣａ２＋ ，Ｍｇ２＋ －ＡＴＰａｓｅ 活性下降，膜蛋白颗粒数减少（Ｐ＜０．０５），组Ⅳ无减少（Ｐ＞ ０．０５）。复灌后，组Ⅳ心功能、Ｃａ２＋ ，Ｍｇ２＋ －ＡＴＰａｓｅ活性恢复正常，Ｎａ＋ ，Ｋ＋ －ＡＴＰａｓｅ 活性和膜蛋白颗粒数恢复稍差。结论：单纯ＣＰＢ对心功能、膜蛋白无损伤作用；温血停搏液心肌保护作用较好，但仍需完善。
Study of cold crystal and warm cardioplegia protective effect on the myocardium. Methods: 96 cats were randomly divided into CPB group (Ⅰ), injury group (Ⅱ), cold crystal protection group (Ⅲ) and warm - protection group (Ⅳ). The changes of cardiac function, membrane ATPase activity and membrane protein granules in each group were observed. Results: There was no change in each index in group Ⅰ at all time points. During ischemia, the activity of Na + and K + -ATPase in group Ⅱ ~ Ⅳ decreased (P <0.01); the activity of group Ⅱ, ⅢCa2 +, Mg2 + -ATPase decreased and the number of membrane protein decreased (P <0.05) (P> 0.05). After reperfusion, the cardiac function, Ca2 +, Mg2 + -ATPase activity of group Ⅳ returned to normal, and the activity of Na +, K + -ATPase and the number of membrane protein particles recovered slightly. CONCLUSION: CPB alone has no effect on cardiac function and membrane protein. Cardioprotection by warm blood cardioplegia is better, but it still needs improvement.