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在不同专化型尖孢镰刀菌(Fusarium oxysporum)的线粒体DNA之间存在着限制性片段长度多态性(RFLP),这可能与它们的致病性差异有关。我们对一株尖孢镰刀菌(F.oxysporum f. sp. eleocharidis1415-1)的线粒体DNA进行酶切分析发现它的酶切图谱和大小与其它专化型的线粒体DNA不同。 1 菌体的培养 用孢子悬浮液接种在蛋白胨-麦芽糖培养基(KH_2PO_4 1％,MgSO_4 7H_2O 1％,蛋白胨0.5％)中,培养4天,用纱布抽滤。 2 线粒体的收集 将菌丝用液氮研磨后,用50mM Tris·HCl(pH8.0),50mM EDTA,15％蔗糖溶
There are restriction fragment length polymorphisms (RFLPs) between mitochondrial DNAs of different Fusarium oxysporum strains, which may be related to their pathogenicity differences. We digested mitochondrial DNA from a strain of F. oxysporum f. Sp. Eleocharidis1415-1 and found that its digestion pattern and size differed from those of other specialized mitochondrial DNAs. Culture of 1 cell strain Spore suspension was inoculated into peptone-maltose medium (KH 2 PO 4 1%, MgSO 4 7H 2 O 1%, peptone 0.5%), cultured for 4 days, and filtered with gauze. 2 Collection of mitochondria The mycelium was ground with liquid nitrogen and then treated with 50 mM Tris.HCl (pH 8.0), 50 mM EDTA, 15% sucrose