THE TRANTHE TRANSMEMBRANE SIGNAL TRANSDUCTION INHEp~(-2) CELLSINDUCED BY BACTERIAL ADHERENCE

• 作 者: 管远志 薛莉 叶菜英 张德昌
• 出 处: Chinese Medical Sciences Journal
• 发表日期: 2000年01期
• 关键词: EPEC stationary incubation medicines picture pathogenic antibiotics steps viewpo

In order to understand the role of transmembrane signal transduction of host cells in the early steps of infection,the adherence of E. coli to HEp 2 cells and the change of activity of phospholipase C γ (PLC γ) induced by the adherence were investigated.The adherence of enteropathogenic E.coli (EPEC), strain E.7, induced a significant increase of inositol triphosphat (IP 3) level in HEp 2 cells. The adherence of the bacteria and the increase of IP 3 was kinetically correlated. Whereas the increase of IP 3 level induced by the adherence of the control strain EPEC (H511), a non piliated strain, was much meager than that by E7, a piliated strain. The results highlighted an important role of transmembrane signals like IP 3 in the pathogenesis of EPEC.

In order to understand the role of transmembrane signal transduction of host cells in the early steps of infection, the adherence of E. coli to HEp 2 cells and the change of activity of phospholipase C γ (PLC γ) induced by the adherence were investigated. The adherence of the enteropathogenic E. coli (EPEC), strain E.7, induced a significant increase of inositol triphosphat (IP 3) level in HEp 2 cells. The adherence of the bacteria and the increase of IP 3 was kinetically correlated. Whereas the increase of IP 3 level induced by the adherence of the control strain EPEC (H511), a non piliated strain, was much meager than that by E7, a piliated strain. The results highlighted an important role of transmembrane signals like IP 3 in the pathogenesis of EPEC.