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自从 Greenberg 等的报告以来,对乙型肝炎的干扰素疗法引起重视。可是作为肝炎病的基础,患者血清中干扰素的动态,所知甚少。作者在肝炎患者中测定了干扰素的效价,以探讨这种病态和病程的关系。对象和方法:把病毒引起的肝病50例作为对象。用空斑减少法测定干扰素的效价,有些病例则合并应用细胞病变抑制法,以确定其效价。空斑减少法,以羊膜细胞作成单层细胞培养,试验时,先吸除培养液,把阶段稀释的血清加入其中,在37℃的冰箱里20小时,并在5%二氧化碳中进行培养,然后加入50～100 P、F、U(空斑形成单位)培养1～2小时,使 V S V(水泡性口腔炎病毒)吸附在细胞上,然后用营养琼脂复盖其上,经
Since the Greenberg et al. Report, attention has been given to interferon therapy for hepatitis B. However, little is known about the dynamics of interferon in the patient’s serum as a basis for hepatitis. The authors measured interferon titer in patients with hepatitis to explore the relationship between this morbidity and course of disease. Subjects and Methods: The virus-induced liver disease in 50 cases as the object. Interferon potency was determined by the plaque reduction assay, and in some cases cytopathic effect inhibition was used in combination to determine its potency. Plaque reduction method, monolayer culture of amniotic cells, the test, the first absorption of the culture medium, the phase dilution of serum added to it in the refrigerator at 37 ℃ for 20 hours and cultured in 5% carbon dioxide, and then Add 50 ~ 100 P, F, U (plaque forming units) for 1 to 2 hours, the VSV (vesicular stomatitis virus) adsorption on the cells, and then covered with nutrient agar by