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在热带地区的水稻栽培中，常遇到水稻青铜病（ｂｒｏｎｚｉｎｇ）的危害．已知它是由水田中高浓度的亚铁离子所引起，故又叫铁害．但至今没有可靠的生理诊断指标用于抗性品种的筛选．本文研究了铁害与应激乙烯释放的关系，试图以应激乙烯的释放作为铁害的生理诊断指标．试验用两种方法模拟水稻致病．第一种方法是将水稻离体叶片的剪口端浸入ＦｅＳＯ４溶液中，靠叶片蒸腾作用吸收Ｆｅ＋＋而致病．另一种方法是在水培培养液中加入ＦｅＳＯ４通过水稻根系吸收Ｆｅ＋＋而致病．研究结果表明，当处理离体叶片时，发病强度和应激乙烯释放量呈显著相关，但叶片内铁含量的增加与发病强度和应激乙烯释放都没有相关性．而处理完全植株时，叶片中乙烯释放几乎不受影响．当部分或全部切除根时，叶片中乙烯释放则可被亚铁离子激发。表明水稻根系限制了Ｆｅ＋＋的吸收速率，而Ｆｅ＋＋进入叶组织的速率又决定应激乙烯的释放和组织的伤害程度．因此，叶片应激乙烯的释放作为铁害的生理诊断指标只有在当根系受到某种伤害时才可能适用，譬如移栽和毒性土壤等因素造成的根系的伤害．
In rice cultivation in the tropics, the dangers of rice bronzing are often encountered. It is known to be caused by the high concentrations of ferrous ions in the paddy fields, so it is also called iron damage. But so far there is no reliable physiological diagnostic index for the screening of resistant varieties. In this paper, we studied the relationship between iron damage and stress ethylene release and tried to use the release of stress ethylene as a physiological diagnostic indicator of iron damage. Two methods were used to simulate rice pathogenicity. The first method is immersed in the cut end of the leaves of paddy rice FeSO4 solution, by the transpiration of leaves to absorb Fe ++ and pathogenic. Another method is to add FeSO4 to the hydroponic culture solution to induce Fe ++ absorption through rice root. The results showed that there was a significant correlation between the severity of stress and ethylene stress, but no correlation between the iron content and the severity of stress and ethylene stress. Ethylene emissions in the leaves are almost unaffected when the complete plants are treated. When some or all of the roots are excised, the release of ethylene in the leaves can be stimulated by ferrous ions. It showed that rice root limited the absorption rate of Fe ++, and the rate of Fe ++ entering the leaf tissue also determined the release of ethylene and tissue damage. Therefore, leaf stress ethylene release as a physiological indicator of iron damage can only be applied when the root system suffers some damage, such as transplanting and toxic soil damage caused by root causes.