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为了探讨CaCl_2缓解聚乙二醇(PEG)胁迫下2种抗旱性不同品种苜蓿幼苗叶片解剖结构的变化,试验采用石蜡切片方法,对2种苜蓿叶片解剖结构进行观察和比较。结果表明:在PEG胁迫后苜蓿叶片厚度、上下表皮厚度、栅栏组织和海绵组织厚度、中脉厚度、栅海比值、叶片栅栏组织结构紧密度(CTR值)和叶片海绵组织结构疏松度(SR值)均有降低趋势;经CaCl_2缓解后这些数据有升高趋势,其中叶片厚度、上下表皮厚度、栅栏组织和海绵组织厚度、中脉厚度等指标差异较大,栅栏组织厚度/海绵组织厚度(栅海比)、CTR值和SR值差别较小;抗旱性强的草原1号苜蓿叶片的栅栏组织较厚,叶片组织紧密度大、疏松度小,栅海比较高,中脉厚度比较大。
In order to investigate the effects of CaCl 2 on the changes of the anatomical structure of leaves of two alfalfa varieties with different drought resistances under polyethylene glycol (PEG) stress, the anatomical structures of the two alfalfa leaves were observed and compared by paraffin section method. The results showed that leaf thickness, upper and lower epidermis thickness, palisade and spongy tissue thickness, midrib thickness, ratio of grate to sea, CTR of leaf palisade and SR value of leaf spongy tissue after PEG stress. These data showed a trend of increasing after CaCl 2 remission. Leaf thickness, thickness of upper and lower epidermis, palisade tissue and spongy tissue thickness, midrib thickness were significantly different, palisade tissue thickness / spongy tissue thickness ). The difference of CTR value and SR value was small. The leaf tissue of Medicago sativa No.1 with stronger drought resistance had thicker palisade tissue, larger leaf tissue density, less looseness, higher ratio of grate to sea, and thicker midrib.