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以萌发初期大豆弯曲的子叶节为靶点,利用草甘膦(Roundup)点施鉴定法,分析了221份大豆微核心种质的草甘膦耐受性及其与抗草甘膦转CP4-EPSPS基因大豆AG5601的差异,结果表明,草甘膦对不同大豆种质的抑制程度与点施的草甘膦浓度呈正相关,Roundup稀释浓度为1/1000、1/10000时,草甘膦对大豆生长几乎无影响;Roundup稀释浓度为1/10时,草甘膦显著抑制大豆生长,导致植株死亡;Roundup稀释浓度为1/100时,不同大豆种质对草甘膦耐受性差异显著,并鉴定出对草甘膦具有较好耐受性的种质10份。虽然大豆微核心种质对草甘膦的耐受程度远远低于AG5601,但不同大豆种质对草甘膦耐受性存在显著差异,这为利用转基因和杂交转育技术培育抗草甘膦转基因大豆的受体或轮回亲本的选择提供了理论依据。
In the early stage of germination, the cotyledon node of soybean was taken as the target, and the glyphosate tolerance of 221 soybean microcarrier germplasm and its relationship with the resistance to glyphosate-transfered CP4- EPSPS gene soybean AG5601 differences, the results showed that glyphosate on the different soybean germplasm and the point of application of glyphosate concentration was a positive correlation, Roundup dilution of 1 / 1000,1 / 10000, glyphosate on soybean Glyphosate significantly inhibited the growth of soybean and led to the death of plant at the roundup dilution of 1/10; the glyphosate tolerance of different soybean germplasms was significant at 1/100 of Roundup dilution and 10 germplasms with good tolerance to glyphosate were identified. Although tolerance to glyphosate in MCC was far lower than that of AG5601, the tolerance of glyphosate to different soybean germplasms was significantly different, which is a potential strategy to develop glyphosate-resistant glyphosate using transgenic and cross-breeding techniques The genetically modified soybean receptor or recurrent parental selection provides a theoretical basis.