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非洲菊(Gerbera jamesonii Bolus)为菊科宿根草本。我们经过多次的试验,成功地对其进行了组织培养。 1.外植体的采集和消毒取较大的未开放的花蕾,用自来水洗净,再用0.1％的升汞处理8分钟,最后用无菌水冲洗4～5次。把花蕾剥开,接种在培养基上。 2。培养基和培养方法培养基为MS+BA1+IBA0.5+糖4％+琼脂9％,pH 5.8,培养温度为27～28℃,光强为2000lux。2周后,花瓣基部开始膨大并逐渐产生愈伤组织。1个月后,愈伤组织逐渐分化出芽。将愈伤组织和芽接种在MS+BA0.5+IBA0.2+糖4％+琼脂9％(pH
Gerbera jamesonii Bolus is a Asteraceae perennial herb. After many trials, we successfully organized it. 1. Collecting and disinfecting explants Larger unopened flower buds are washed with tap water and then treated with 0.1% mercuric chloride for 8 minutes. Finally, the cells are washed 4 to 5 times with sterile water. Peel buds, inoculated on the medium. 2. Medium and culture medium: MS + BA1 + IBA0.5 + sugar 4% + agar 9%, pH 5.8, culture temperature 27 ~ 28 ℃, light intensity 2000lux. After 2 weeks, the basal petals began to dilate and gradually produce callus. After 1 month, the callus gradually differentiated budding. Callus and buds were inoculated on MS + BA0.5 + IBA0.2 + sugar 4% + agar 9% (pH