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目的研究α-干扰素和环氧合酶(COX-2)抑制剂塞来昔布(Celecoxib)对人肝癌SMMC-7721细胞的生长抑制作用。方法采用MTT比色法观察不同浓度的塞来昔布和α-干扰素处理对肝癌细胞增殖的影响;通过荧光显微镜检测细胞凋亡。结果实验组与对照组相比,能抑制人肝癌SMMC-7721细胞的增殖,两种药物高浓度联合作用48 h后,对肝癌细胞的生长抑制率可达92.44%±0.98%。α-干扰素和塞来昔布对肝癌细胞的增殖抑制具有协同作用。荧光显微镜观察到细胞皱缩、核质浓缩、核碎裂以及凋亡小体形成等凋亡形态学改变。结论 COX-2抑制剂塞来昔布和α-干扰素对人肝细胞癌SMMC-7721细胞的增殖有协同抑制作用,同时能诱导其凋亡,并且呈剂量和时间依赖性,这提示α-干扰素和COX-2抑制剂塞来昔布对HCC的预防和治疗可能有积极意义。
Objective To study the inhibitory effect of Celecoxib, an inhibitor of cyclooxygenase and cyclooxygenase (COX-2), on human hepatoma SMMC-7721 cells. Methods MTT assay was used to observe the effects of different concentrations of celecoxib and α-interferon on the proliferation of hepatocellular carcinoma cells. The apoptosis of hepatocellular carcinoma cells was detected by fluorescence microscope. Results Compared with the control group, the experimental group inhibited the proliferation of human hepatocellular carcinoma SMMC-7721 cells. The inhibitory rate of hepatocellular carcinoma cells was 92.44% ± 0.98% after 48 h of high concentration of two drugs. Interferon-alpha and celecoxib have a synergistic effect on the inhibition of proliferation of hepatoma cells. Fluorescence microscopy showed morphological changes of apoptosis such as cell shrinkage, condensation of nuclei, nuclear fragmentation and formation of apoptotic bodies. Conclusions COX-2 inhibitor celecoxib and α-interferon have a synergistic inhibitory effect on the proliferation of human hepatocellular carcinoma SMMC-7721 cells and induce their apoptosis in a dose- and time-dependent manner, suggesting that α- Interferon and COX-2 inhibitor celecoxib may have positive implications for the prevention and treatment of HCC.