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目的观察黄芪注射液对大鼠肾脏缺血再灌注损伤的保护作用及机制。方法将SD大鼠随机分为假手术组、模型组、黄芪注射液组、维生素E组、黄芪+维生素E组,每组15只,采用切除右肾、动脉夹夹闭左肾动脉1h后松开恢复血流方法复制肾缺血再灌注损伤动物模型。黄芪注射液组、维生素E组分别于术前1h腹腔注射黄芪注射液10mg/kg或维生素E100mg/kg,黄芪+维生素E组同时给予上述两种药物。于缺血1h,再灌注1h、24h分别检测各组血清中尿素氮(BUN)、肌酐(Cr)含量、尿液中肾损伤分子-1(KIM-1)、6-乙酰-β-D-氨基葡萄糖酐酶(NAG)、肾组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)水平,并观察肾组织HE染色病理改变。结果与模型组比较,各治疗组血清BUN、Cr水平均显著降低(P<0.05或P<0.01),尿液KIM-1、NAG水平均显著降低(P<0.05或P<0.01),肾组织匀浆SOD活力升高、MDA含量降低(P<0.05或P<0.01),各治疗组之间比较差异有统计学意义(P<0.05)。肾组织切片镜下可见模型组大量肾小管上皮细胞肿胀、坏死,部分肾小管腔内可见蛋白性液体、红细胞或细胞碎片,治疗组肾组织病变减轻较明显。结论黄芪注射液和维生素E均可以增强SOD活性,清除氧自由基,减轻脂质过氧化作用,对缺血再灌注肾脏具有保护作用。黄芪注射液作用优于维生素E,二者联用效果更好。
Objective To observe the protective effect of Astragalus injection on renal ischemia-reperfusion injury in rats and its mechanism. Methods SD rats were randomly divided into sham operation group, model group, astragalus injection group, vitamin E group, astragalus + vitamin E group, 15 rats in each group. Restoration of blood flow in the replication of renal ischemia-reperfusion injury in animal models. Astragalus injection group and vitamin E group were given intraperitoneal injection of astragalus injection 10mg / kg or vitamin E100mg / kg 1h before operation, and the two drugs were administered simultaneously with Astragalus + Vitamin E group. Serum urea nitrogen (BUN), creatinine (Cr), urine KIM-1, 6-acetyl-β-D- (NAG), the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) in renal tissue were observed. The pathological changes of HE staining were observed. Results Compared with the model group, the serum levels of BUN and Cr were significantly decreased (P <0.05 or P <0.01) and the levels of KIM-1 and NAG in urine were significantly decreased (P <0.05 or P <0.01) The homogenized SOD activity increased and MDA content decreased (P <0.05 or P <0.01). There was significant difference between each treatment group (P <0.05). In the renal tissue section, a large number of renal tubular epithelial cells were swollen and necrotic in the model group, and protein liquid, erythrocytes or cell debris were seen in some of the renal tubular cavities. The lesion in the treated group was more obvious than that in the untreated group. Conclusion Astragalus injection and vitamin E can enhance SOD activity, scavenge oxygen free radicals and reduce lipid peroxidation, and have a protective effect on kidney after ischemia-reperfusion. Astragalus injection is better than vitamin E, the combination effect is better.