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目的对2008～2009年濮阳市辖区内手足口病住院患者进行病原体核酸检测。方法用RT-PCR法对306例手足口病住院患者的咽拭子、疱疹液和血清样本进行肠道病毒、EV71、CoxA16核酸检测。结果 EV71病毒核酸阳性率为16.25%,CA16病毒核酸阳性率为0.28%。PE单独(非EV71、CA16的其它肠道病毒)阳性率为61.4%。疱疹液阳性率最高为73.3%,粪便阳性率为58.3%,咽拭子阳性率为40.7%,血清阳性率最低为4.35%,咽拭子与粪便阳性率无差异。病例以6岁以下为主,占97.06%。2008年有45例,EV71肠道病毒核酸阳性率为26.67%。2009年有261例,EV71阳性率为11.11%,CA16检出1例,PE单独阳性率为29.50%。结论 2009年与2008年相比EV71外其它肠道病毒引起手足口病感染的概率在增加。咽拭子标本采集方法简单易掌握,能达到早采样早诊断早治疗的目的,要加大对6岁以下儿童的监测检测力度,为控制手足口病疫情提供科学依据。
Objective To detect pathogen nucleic acid in hospitalized patients with HFMD in Puyang City from 2008 to 2009. Methods RT-PCR was used to detect enterovirus, EV71 and CoxA16 in throat swab, herpes fluid and serum of 306 HFMD patients. Results The positive rate of nucleic acid of EV71 virus was 16.25% and the positive rate of CA16 virus nucleic acid was 0.28%. The positive rate of PE alone (other enterovirus other than EV71 and CA16) was 61.4%. The positive rate of herpes fluid was 73.3%, the positive rate of stool was 58.3%, the positive rate of throat swab was 40.7%, and the lowest positive rate of serum was 4.35%. The positive rate of throat swab and stool was no difference. The cases were mainly under 6 years old, accounting for 97.06%. 45 cases in 2008, EV71 enterovirus nucleic acid positive rate was 26.67%. There were 261 cases in 2009, the positive rate of EV71 was 11.11%, and one case of CA16 was detected. The positive rate of PE alone was 29.50%. Conclusion The incidence of HFMV infection among other enteroviruses outside EV71 in 2009 is increasing. Throat swab specimen collection method is simple and easy to grasp, can achieve the early diagnosis and early treatment of early sampling purposes, to increase the detection and detection of children under 6 years of age, to provide a scientific basis for the control of HFMD outbreak.