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为了解利用母血循环中胎儿单拷贝基因的灵敏度并初步探索其临床应用价值,本研究根据人类SRY基因保守顺序设计了内、外两对引物,在明确该检测灵敏度的基础上,对15例孕妇血中胎儿SRY基因进行了扩增,结果表明这一检测程序的灵敏度达到1/2×10~7(男性DNA/女性DNA)15例母血胎儿细胞SRY基因的检出率与胎儿实际性别的符合率为93.3％。本实验采取的套式PCR技术大大提高了检测的特异性和灵敏度。由此提示合适的引物设计并采用套式PCR技术可提高检测的特异性及灵敏度。
In order to make use of the sensitivity of fetus single copy gene in maternal blood circulation and to explore its clinical application value, two pairs of primers were designed according to the conserved sequence of human SRY gene. Based on the sensitivity of this test, 15 pregnant women The results showed that the detection rate of SRY gene in fetal blood was 15% with the sensitivity of 1 × 2 × 10 ~ 7 (male DNA / female DNA) and the actual sex of fetus The coincidence rate was 93.3%. The nested PCR technique adopted in this experiment has greatly improved the specificity and sensitivity of detection. This suggests that the appropriate primer design and use of nested PCR technology can improve the detection specificity and sensitivity.