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目的应用荧光原位杂交(FISH)技术研究肺癌患者染色体数目改变及其意义。方法用人的全套染色体特异探针与肺癌患者中期染色体进行杂交检测。结果肺癌患者细胞为异倍体,染色体数目以亚二倍体居多,常见2、5、7、8、10、11、14和17号染色体增多及1、4号染色体的丢失。结论 FISH技术可检测肺癌患者染色体数目的改变,肺癌遗传学变异主要是2、5、7、8、10、11、14、17号染色体的增加,并对肺癌的发生、发展和预后有一定的提示作用。
Objective To investigate the change of chromosome number in patients with lung cancer by fluorescence in situ hybridization (FISH) and its significance. Methods A full set of chromosome-specific probes was used to detect metaphase chromosomes in patients with lung cancer. Results The number of lung cancer cells was aneuploid, the number of chromosomes was mostly subdued, and chromosomes 2, 5, 7, 8, 10, 11, 14 and 17 were common and the chromosomes 1 and 4 were lost. Conclusions FISH can detect the number of chromosome changes in patients with lung cancer. The genetic variation of lung cancer is mainly the increase of chromosomes 2, 5, 7, 8, 10, 11, 14 and 17, and has certain effects on the occurrence, development and prognosis of lung cancer Prompt effect.