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目的研究人脱细胞膀胱黏膜下基质(AUBS)异种移植的转归及免疫原性。方法将人AUBS异种移植于SD大鼠的腹壁肌肉内,分别于术后3、6、10、14、21和28 d取材进行组织学观察和FITC标记的山羊抗大鼠IgG免疫荧光染色观察。将人脱细胞真皮基质和未脱细胞膀胱黏膜下层作为对照进行对比观察。结果人AUBS可以诱导组织细胞浸润、生长,14 d内基质移植区血管化,28 d内移植基质被机体降解吸收。IgG免疫荧光染色观察显示,基质移植后局部无明显IgG免疫复合物的沉积。结论人AUBS植入大鼠腹壁肌肉后可以诱导组织细胞长入和血管化,并在新生组织形成过程中迅速降解吸收,不引起明显的免疫反应。
Objective To study the prognosis and immunogenicity of human acellular bladder submucosal (AUBS) xenografts. Methods Human AUBS was transplanted into the abdominal muscles of SD rats. Histological observation and FITC labeled goat anti-rat IgG immunofluorescence staining were performed on the 3rd, 6th, 10th, 14th, 21st and 28th postoperative days respectively. Human acellular dermal matrix and non-decellularized bladder submucosa were compared as a control. Results AUBS could induce cell infiltration and growth of the cells, and vascularization of stromal transplantation area within 14 days. The transplanted stroma was degraded and absorbed by the body within 28 days. IgG immunofluorescence staining showed no significant deposition of IgG immunocomplex in the area after the transplantation of the matrix. Conclusion Human AUBS implanted rat abdominal wall muscles can induce the growth of cells and vascularization, and rapid degradation during the formation of new tissue absorption, does not cause a significant immune response.