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实时荧光定量PCR(q RT-PCR)具有灵敏度高、特异性强、重复的动态定量范围和高通量等优点,是进行植物基因定量分析最常用的技术手段之一。番茄黄化曲叶病毒病(tomato yellow leaf curl virus disease,TYLCD)是番茄生产上最严重的病害,为了研究番茄对于TYLCD的抗性与病毒含量之间的相关性,本研究利用q RT-PCR技术对含有不同抗病基因番茄材料、感病番茄材料中病毒的含量进行分析。结果表明:接种后同一时期抗病材料的病毒含量总体低于感病材料;除Ty-1+2+3+5番茄材料外,其它抗病番茄材料随着时间的增长,病毒含量均呈现先升高后降低的趋势,有效地阻止了病毒繁殖;番茄材料的病毒含量与抗性呈现负相关性。本研究可为进一步研究和导入新的抗病基因提供理论指导。
Real-time quantitative PCR (q RT-PCR) has the advantages of high sensitivity, strong specificity, repeated dynamic range and high throughput. It is one of the most commonly used techniques for quantitative analysis of plant genes. Tomato yellow leaf curl virus disease (TYLCD) is the most serious disease in tomato production. In order to study the correlation between tomato resistance to TYLCD and virus content, we used q RT-PCR The technique analyzes the content of virus in tomato material containing different disease-resistance genes and susceptible tomato materials. The results showed that the virus content of the disease-resistant material was generally lower than that of the susceptible material during the same period after inoculation. The content of virus in the disease-resistant tomato material increased with time except the Ty-1 + 2 + 3 + 5 tomato material Increased and then decreased, which effectively prevented the virus from multiplying. The content of virus in tomato material was negatively correlated with the resistance. This study can provide theoretical guidance for further research and introduction of new resistance genes.