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目的观察补充碘剂对自身免疫性甲状腺病大鼠甲状腺功能的影响。方法采用皮下注射甲状腺球蛋白诱发实验性自身免疫性甲状腺炎动物模型(EAT),通过饮水中给予碘剂观察对大鼠甲状腺重量、形态学改变、促甲状腺激素(TSH)和甲状腺激素谱以及甲状腺自身抗体滴度变化。雌性Wisrar大鼠分为A空白对照组、B抗原免疫组、C碘剂喂养组及D抗原免疫加碘剂组。免疫组大鼠给予猪甲状腺球蛋白100μg/只,与全弗氏佐剂(CFA)乳化后皮下注射,第2周后给予不完全弗氏佐剂(IFA)乳化抗原加强免疫。碘剂干预组给予0.05%碘化钠溶液喂养。分别于第0,2,4,6,8周内眦静脉采集血样。结果D组甲状腺绝对及相对重量显著高于A、B组。P<0.05,略高于C组;C组高于A、B组,但P>0.05。B组小叶间散见淋巴细胞浸润;碘剂组滤泡异质性改变,小叶间少量淋巴细胞浸润;D组甲状腺滤泡灶性萎缩、炎症细胞浸润明显,范围较广,滤泡周围可见纤维组织增生。第8周B组FT3、FT4高于A、B组;D组升高更为显著。P<0.01。B组大鼠血清TgAb、TmAb自第2周开始升高,至第4周达到最高水平,随后略有下降,但仍然显著高于对照组;C组抗体水平缓慢升高,第8周时升高达到高峰;D组抗体水平升高最快,而且维持时间更长,P<0.01。结论碘剂可以加重EAT大鼠甲状腺病理损害,升高甲状腺自身抗体滴度,
Objective To observe the effects of iodine supplementation on thyroid function in autoimmune thyroiditis rats. Methods The model of experimental autoimmune thyroiditis (EAT) was induced by subcutaneous injection of thyroglobulin, and the thyroid weight, morphological changes, thyroid stimulating hormone (TSH) and thyroid hormones and thyroid Autoantibody titer changes. Female Wisrar rats were divided into A blank control group, B antigen immune group, C iodine-fed group and D antigen immune iodine group. The immunized rats were given 100 μg porcine thyroglobulin, subcutaneously injected with whole Freund’s adjuvant (CFA), and boosted with incomplete Freund’s adjuvant (IFA) emulsified antigen after 2 weeks. Iodine intervention group was given 0.05% sodium iodide solution. Blood samples were taken from 眦 vein in 0, 2, 4, 6, 8 weeks respectively. Results The absolute and relative thyroid weights of group D were significantly higher than those of group A and B. P <0.05, slightly higher than C group; C group was higher than A, B group, but P> 0.05. B group of scattered lobular lobular lymphocyte infiltration; iodine group of follicular heterogeneity change, a small amount of lymphocyte infiltration between lobes; D group follicular thyroid atrophy, inflammatory cell infiltration was obvious, a wide range of fibrous tissue around the follicles Tissue hyperplasia. In the 8th week, the FT3 and FT4 in group B were higher than those in group A and B; the increase in group D was more significant. P <0.01. The serum levels of TgAb and TmAb in group B increased from the second week to the highest level in the fourth week and then decreased slightly but still significantly higher than those in the control group. The antibody level in group C increased slowly and reached the level of the eighth week Peak; D group antibody levels increased fastest, and maintain a longer time, P <0.01. Conclusion Iodine can aggravate thyroid pathological damage in EAT rats and increase thyroid autoantibody titers,