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目的：为探索烷化剂对哺乳动物早期胚胎发育里程的影响及其可能的机制。方法：孕大鼠第３ 天ｉｐ 环磷酰胺（Ｃｙｃ １０ 、２０ 、４０ｍｇ／ｋｇ） ，孕第４ 天评价胚胎的胚胎化作用，胚胎发育时相，及蜕变胚胎率；同时显微外科分离胚胎内细胞团及滋养层细胞，评价两群细胞受损情况。结果：在Ｃｙｃ １０ｍｇ／ｋｇ 时，胚胎的胚泡化作用，胚胎发育时相均未受明显影响；４０ｍｇ／ｋｇ 时，蜕变胚胎率明显提高。胚泡化率及胚胎细胞数呈剂量依赖性减少，其中内细胞团细胞减少尤甚。结论：Ｃｙｃ 在未引起早期胚胎蜕变率显著增加时，可影响细胞周期，但不影响发育里程；高剂量时，显著影响发育里程并伴蜕变率显著增加
Objective: To explore the effect of alkylating agents on mammalian early embryonic developmental distance and its possible mechanism. Methods: The pregnant rats were treated with ip cyclophosphamide (Cyc 10, 20, 40 mg / kg) on day 3, embryo embryogenesis, embryonic development phase and the rate of embryo transformation on the 4th day of pregnancy. Simultaneously, Within the cell mass and trophoblast cells, evaluation of two groups of cell damage. Results: At 10 mg / kg Cyc, embryo blastocystization and embryo development were not significantly affected. At 40 mg / kg, the rate of embryo transformation was significantly increased. The rate of blastocyst and the number of embryo cells decreased in a dose-dependent manner, especially in the inner cell mass. Conclusion: Cyc did not affect the cell cycle but did not affect the developmental distance when not significantly increasing the rate of early embryo degeneration. At high dose, Cyc significantly affected the developmental distance and the rate of metamorphosis was significantly increased