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在泰国,大劣按蚊复合体由形态相同的近似种A,B,C,D和An.nemophilus组成。以往是通过染色体核型或同工酶电泳鉴定这些蚊种,但难以在野外应用。利用DNA探针鉴定近似种有很高的特异性,但同位素对人体有危害,并可污染环境。作者用辣根过氧化酶(HRP)标记DNA探针,以化学发光检测系统鉴定大劣按蚊近似种,具有敏感性高,可靠性强、快速、适于野外操作等优点。 用于鉴定大劣按蚊A,B,C和D的特异性探针分别为PMu-A40.1＃5,PMu-B5,
In Thailand, the Anopheles dirus complex consists of the closely related species A, B, C, D and An.nemophilus. In the past, these mosquitoes were identified by chromosomal karyotype or isoenzyme electrophoresis, but were difficult to apply in the wild. The use of DNA probes to identify the approximate species has a high specificity, but isotopes are harmful to humans and can pollute the environment. Using horseradish peroxidase (HRP) -labeled DNA probes, the chemiluminescence detection system was used to identify the species of Anopheles dirus, which has the advantages of high sensitivity, high reliability, fastness and suitable for field operation. The specific probes used to identify Anopheles dirus A, B, C and D were PMu-A40.1 # 5, PMu-B5,