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用宁夏枸杞－ 宁杞１ 号的髓组织进行离体培养，诱导产生胚性愈伤组织，并用６０Ｃｏγ射线进行诱变，以枸杞根腐菌尖孢镰刀菌（ Ｆｕｓａｒｉｕｍ ｏｘｙｓｐｏｒｕｍ Ｓｃｈｌｅｃｈｔ） 菌株产生的培养滤液为选择剂，筛选抗性变异体，在含毒素６０ ％ 的条件下，一次性获得抗根腐病毒素的愈伤组织，抗性愈伤组织经稳定性分析，其抗性稳定。抗性愈伤组织在含３０ ％ 的毒素培养基中可分化成再生植株，共获得２５ 株，其叶片经用根腐菌分生孢子液接种鉴定，有６ 株抗病；再生植株经过脯氨酸、叶绿素含量分析，二者含量都有所提高；经过过氧化物同工酶酶谱分析，抗性再生植株酶谱带数有增加；说明经筛选得到的抗性再生植株是抗根腐病的变异体
The medullary tissue of Ningxia wolfberry - Ningqi 1 was used for in vitro culture to induce the embryogenic callus, which was induced by 60 Coγ-ray and was produced by Fusarium oxysporum Schlecht strain The filtrate was selected as a selective agent to screen for resistant variants. The calluses resistant to root rot virus were obtained once under the condition of containing 60% toxin, and the resistant callus was analyzed for stability and the resistance was stable. Resistant calli could be differentiated into regenerated plants in 30% of toxin medium, 25 of them were obtained. The leaves were identified by the inoculation of root rot fungi conidia, and 6 of them were resistant to disease. Acid and chlorophyll content, both of which increased. After peroxidase isozyme zymogram analysis, the number of bands of the resistant regenerated plants increased. It indicated that the resistant regenerated plants were resistant to root rot Of the variants