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目的 :探讨在原发性食管鳞癌组织中染色体 9pter～ p2 1区域内的杂合性缺失及其意义。方法 :采用微卫星序列 PCR银染方法 ,检测 9pter～ p2 1区域内 3个位点的 DNA微卫星多态标记 ,并对 43例散发性原发食管鳞癌标本及其癌旁正常组织进行杂合性缺失分析。结果 :9pter～ p2 1区域内 3个 DNA微卫星多态标记的杂合性缺失率均大于 5 0 % ,有显著意义。结论 :在染色体 9pter～ p2 1区域内存在与食管鳞癌密切相关的抑癌基因
Objective: To investigate the loss of heterozygosity in chromosome 9 plex ~ p2 1 in primary esophageal squamous cell carcinoma and its significance. Methods: Microsatellite DNA microsatellite PCR was used to detect DNA microsatellite polymorphism at three loci in 9 plex ~ p2 1 region. 43 cases of sporadic primary esophageal squamous cell carcinoma and its adjacent normal tissues were heterozygous Coincidence loss analysis. Results: The loss rate of heterozygosity of 3 DNA microsatellite polymorphisms in 9 plex ~ p2 1 region was more than 50%, which was significant. Conclusion: There is a tumor suppressor gene closely related to esophageal squamous cell carcinoma in chromosome 9 plex ~ p2 1 region