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本法基于经碱比后的血浆用正戊烷-异丙醇(50:1)提取三氟丙拉嗪(Trifluoperazine)和甲哌氯丙嗪内标,然后将提取物中的有机溶剂蒸发至干。残留物用少量甲醇溶解供注射进样。用NPD可检出血浆中0.5ng/ml的三氟丙拉嗪。血浆中三氟丙拉嗪浓度在0.5～1.5ng/ml范围内呈线性关系。平均斜率值为0.1003×+0.0035(r~2=0.9996)。样品测定的变异系数为5.3%。测得结果与GLC-MS法接近一致。每样品色谱分析时间仅需2分钟。
This method is based on the extraction of trifluoro-Trifluoperazine and prochlorperazine promethazine internal standard with n-pentane-isopropanol (50: 1) from an alkali-stained plasma and then evaporation of the organic solvent in the extract to dry. The residue is dissolved in a small amount of methanol for injection injection. NPD was used to detect 0.5 ng / ml of trifluoroperazine in plasma. Plasma concentrations of trifluoroperazine in the range of 0.5 ~ 1.5ng / ml showed a linear relationship. The average slope is 0.1003 × + 0.0035 (r ~ 2 = 0.9996). The coefficient of variation of the sample was 5.3%. The measured results are in agreement with the GLC-MS method. Chromatographic analysis takes only 2 minutes per sample.