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目的:研究复方丹参滴丸干预冠心病血瘀证凋亡相关基因,寻找复方丹参滴丸干预CHD血瘀证的有效靶点。方法:通过中西医结合病证临床研究,用逆转录-聚合酶链反应(RT-PCR)方法,观察常规治疗对照组治疗前后和复方丹参滴丸治疗组治疗前后凋亡相关基因的变化。结果:治疗组治疗后B淋巴细胞瘤-2(Bcl-2)Associated Transcription Factor1(BCLF1)mRNA表达显著降低(P<0.01),凋亡相关基因(Bcl-2 assaciated X protein,Bax)mRNA表达有降低趋势,两组治疗前后Bcl-2和凋亡信号转导分子(Cysteine asparate specific protease-3,Caspase-3)mRNA表达无明显差异。结论:复方丹参滴丸治疗后冠心病血瘀证患者相关基因凋亡相关转录因子BCLF1 mRNA表达显著减弱,凋亡相关基因Bax mRNA表达受到抑制,复方丹参滴丸可能通过调控细胞凋亡相关基因的表达,对冠心病血瘀证患者发挥治疗保护作用。
Objective: To study the effect of compound Danshen dripping pills on the apoptosis-related genes of blood stasis syndrome of coronary heart disease (CHD), in order to find out the effective target of compound Danshen dripping pills in the treatment of CHD blood stasis syndrome. Methods: The changes of apoptosis-related genes in the control group before and after treatment and the compound Danshen Dripping Pills group before and after treatment were observed by reverse transcription-polymerase chain reaction (RT-PCR). Results: The expression of BCLF1 mRNA in Bcl-2 significantly decreased (P <0.01), and the expression of Bcl-2 mRNA (P <0.05) .There was no significant difference between the two groups in the mRNA expression of Bcl-2 and Cysteine asparate specific protease-3 before and after treatment. Conclusion: The expression of BCLF1 mRNA of apoptosis-related transcription factor in patients with coronary heart disease and blood stasis syndrome was significantly decreased after treatment with Fufang Danshen Diwan and the expression of apoptosis-related gene Bax mRNA was inhibited. Fufang Danshen Diwan could inhibit the expression of BCLF1, Expression, play a protective role in patients with coronary heart disease and blood stasis.