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用膜片箝技术的细胞贴附式和内面向外式，在机械分离的新生ＳＤ大鼠的大脑皮层神经元上，记录到ＡＴＰ激活的离子通道。此通道的电导为３２ｐＳ，对Ｎａ~＋，Ｋ~＋和Ｃｓ~＋无选择性通透，而对Ｃｌ~－不通透。通道开放时间分布直方图多数需用双指数拟合，少数可用单指数拟合；通道关闭时间分布直方图均需用双指数拟合。通道的平均开放时间和开放概率均不依赖于膜电位；但通道的开放概率随着激动剂ＡＴＰ浓度的增加而增大。当电极内液无ＡＴＰ时，无通道电流。六烃季胶和美加明不能阻断此通道。上述结果表明，新生大鼠的大脑皮层神经元胞体可能存在ＡＴＰ激活的离子通道。
Using patch-clamp cell attachment and inwards-facing outward, ATP-activated ion channels were recorded on neurons isolated from mechanically isolated neonatal SD rats. The conductance of this channel is 32 pS, which is non-selective to Na ~ +, K ~ + and Cs ~ +, but not to Cl ~ -. Channel opening time distribution histogram mostly need double exponential fitting, a few can be used single exponential fitting; channel closure time distribution histogram are required to use double exponential fitting. The average open time and open probability of the channel are independent of the membrane potential; however, the open probability of the channel increases with the increase of the ATP concentration of the agonist. There is no channel current when there is no ATP in the electrode. Six hydrocarbon gelatin and methicillin can not block this channel. The above results show that neonatal rat cerebral cortex neuronal somatic ATP-activated ion channels may exist.