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目的 建立一种病变稳定、制作周期短、重复性好、最接近人类肾小球硬化病理改变的动物模型。方法 在摘除一侧肾脏的基础上 ,通过两次尾静脉注射柔红霉素 ,定期观察血、尿及肾脏组织病理学改变。结果 模型组大鼠于用药后第 4周时 ,出现水肿、大量蛋白尿、低蛋白血症、高脂血症 ,病理形态上开始出现典型的节段性肾小球硬化 ;第 9周时血清肌酐、尿素氮水平显著升高 (P<0 .0 5 ) ,80 %的肾小球出现节段性硬化。结论 此模型制作周期短 ,各项指标均符合肾小球硬化的病理及临床特征。
Objective To establish an animal model with stable lesions, short production cycle, good repeatability and closest pathological changes in human glomerular sclerosis. Methods On the basis of removal of the kidney on one side, daunorubicin was injected twice through the tail vein to observe the histopathological changes of blood, urine and kidney. Results At 4 weeks after treatment, rats in the model group developed edema, massive proteinuria, hypoproteinemia and hyperlipidemia, and typical segmental glomerulosclerosis began to appear on the pathological morphology. Serum Creatinine, urea nitrogen levels were significantly increased (P <0. 05), 80% of the glomerular segmental sclerosis. Conclusion This model has a short production cycle and all the indexes are in accordance with the pathological and clinical features of glomerulosclerosis.