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为明确拮抗放线菌Sy11菌株在预防马铃薯晚疫病方面的潜力,以及Sy11菌株与致病疫霉间的感应现象,采用打孔法和离体组织培养等方法检测Sy11菌株对致病疫霉的抑制和离体生防效果,通过对峙培养法和纸板隔离法明确菌株间的感应现象以及由Sy11菌株感应的致病疫霉信号分子的溶解性和稳定性。结果表明,Sy11菌株活体和菌液对致病疫霉菌丝体生长的抑制率分别为89.7%和82.5%,菌液在马铃薯离体块茎和叶片上的相对保护率均高于85.0%;此外Sy11菌株只有在致病疫霉的诱导作用下才分泌抑菌物质,诱导后产生并分泌的抑菌物质的抑菌率达76.5%;Sy11菌株感应的致病疫霉信号分子易溶于甲醇,可耐受80℃高温。以上结果表明Sy11菌株在预防马铃薯晚疫病方面有较大潜力。本研究为今后进一步分离纯化致病疫霉信号分子,明确感应诱导作用机理提供参考依据。
In order to clearly antagonize the potential of actinomycete strain Sy11 in preventing potato late blight and the induction phenomenon between Sy11 and Phytophthora infestans, the method of puncturing and in vitro tissue culture was used to detect the effect of Sy11 strain on Phytophthora infestans Inhibition and in vitro biocontrol effect. The confrontation culture method and cardboard isolation method were used to clarify the induction phenomenon among strains and the solubility and stability of Phytophthora infestans signal molecules induced by Sy11 strain. The results showed that the inhibitory rates of Sy11 strain on the mycelial growth of Phytophthora infestans were 89.7% and 82.5%, respectively. The relative protection rates of Bacillus licheniformis in vitro on tubers and leaves of potato were higher than 85.0% Only when induced by Phytophthora infestans, the antibacterial substance was secreted by the strain and the inhibitory rate of the antibacterial substance produced and secreted by the strain was 76.5%. The Phytophthora infestans signal molecule induced by Sy11 strain was easily soluble in methanol. Tolerance 80 ℃ high temperature. The above results indicate that Sy11 strain has great potential in preventing late blight of potato. This study provides a reference for further isolation and purification of Phytophthora infestans signal molecules in the future, and a clear induction induction mechanism.