论文部分内容阅读
本文用短波紫外线（ＵＶＣ）照射人胎盘和小牛胸腺ＤＮＡ取得ＵＶ－ＤＮＡ（紫外线导致变性的ＤＮＡ），并分别对新西兰家兔进行长达９周的免疫。结果表明：人胎盘ＵＶ－ＤＮＡ未能引起免疫应答，其抗ＵＶ－ＤＮＡ未能检出。而小牛胸腺的抗ＵＶ－ＤＮＡ的效价为１：８。用兔抗小牛胸腺ＵＶ－ＤＮＡ检测３２例红斑狼疮（ＳＬＥ）病人和２１例正常人的血清，发现ＳＬＥ病人血清中ＵＶ－ＤＮＡ的阳性率为６５．６％，正常人为５％。两者的差异有非常显著性意义（Ｐ＜０．０１）。提示血清中ＵＶ－ＤＮＡ可望成为ＳＬＥ诊断和疗效观察及人群接触紫外线时健康监测的特异和敏感指标之一。
In this paper, UV-DNA (UV-induced denatured DNA) is obtained by irradiating human placenta and calf thymus DNA with shortwave ultraviolet (UVC) light and immunized New Zealand rabbits for up to 9 weeks. The results showed that UV-DNA of human placenta failed to induce immune response and its anti-UV-DNA could not be detected. The calf thymus anti-UV-DNA titer of 1: 8. Serum samples of 32 patients with SLE and 21 normal controls were detected by UV-DNA with rabbit anti-calf thymus. The positive rate of UV-DNA in serum of SLE patients was 65.6% and that of normal persons was 5%. The difference between the two has a very significant (P <0.01). It is suggested that the serum UV-DNA is expected to become one of the specific and sensitive indicators for SLE diagnosis and therapeutic effect observation and health monitoring in the population exposed to ultraviolet light.