Polymorphism of human cytomegalovirus(HCMV) UL144 gene in low passage clinical isolates

• 作 者: Xingxing Fu Liping Li Chaoyang Huang Xiaoman Li Huadong Wang Zhenyuan Xie Ze Chen
• 机 构: Shanghai Institute of Biological Products,Institute of Pediatric Research, Hunan Children’s Hospital
• 出 处: Chinese Science Bulletin
• 发表日期: 2014年25期
• 关键词: genotype isolates Polymorphism congenital sequenced geographic pediatric passage

To explore the impact of gene polymorphism of human cytomegalovirus(HCMV)on virus virulence,the full-length UL144 gene and partial sequence of glycoprotein B(UL55)gene were sequenced and analyzed for 23clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection.Among the 23 isolates,13(57%)were UL144genotype 1A,3(13%)were UL144 genotypes 2 and 7(30%)were UL144 genotype 3;geographic differences in genotype distribution were found for both UL144 and gB gene.No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US.Our results also demonstrated that for all clinical strains of gB genotypes I,III and UL144genotypes 1A,2,and 3 found in this study,mother-to-fetus vertical transmission was possible.

To explore the impact of gene polymorphism of human cytomegalovirus (HCMV) on virus virulence, the full-length UL144 gene and partial sequence of glycoprotein B (UL55) genes were sequenced and analyzed for 23 clinical data of HCMV isolated from urine samples of pediatric patients with 14 (57%) were UL144 genotype 1A, 3 (13%) were UL144 genotypes 2 and 7 (30%) were UL144 genotype 3; geographic differences in genotype distribution were found for both UL144 and gB gene. No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US. Our results also demonstrate that for all clinical strains of gB genotypes I, III and UL144 genotypes 1A, 2, and 3 found in this study, mother-to-fetus vertical transmission was possible.