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AIM:To evaluate the effect of aclacinomycin A-loadedmagnetic polybutylcyanoacrylate nanoparticles on gastrictumor growth in vivo and in vitro.METHODS:Magnetic polybutylcyanoacrylate (PBCA)nanospheres encapsulated with aclacinomycin A (MPNS-ACM) were prepared by interracial polymerization.Particlesize,shape and drug content were examined.FemaleBABL/c nude mice were implanted with MKN-45 gastriccarcinoma tissues subcutaneously to establish humangastric carcinoma model.The mice were randomly dividedinto 5 groups of 6 each:ACM group (8 mg/kg bm);groupof high dosage of MPNS-ACM (8 mg/kg bin);group oflow dosage of MPNS-ACM (1.6 mg/kg bin);group ofmagnetic PBCA nanosphere (MPNS) and control group(normal saline).Magnets (2.5 T) were implanted into thetumor masses in all of the mice one day before the therapy.Above-mentioned drugs were administered intravenouslyto the mice of every group on the first day and sixth day.When the mice were sacrificed,tumor weight wasmeasured,and the assay of granulocyte- macrophagecolony forming-unit (CFU-GM) was performed on semi-solid culture.White blood cell,alanine aminotransferaseand creatine were examined.3-[4-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliurn bromide (MTT) was used toexamine the viability of MKN-45 cells after incubation withdifferent concentrations of ACM,MPNS and MPNS-ACMsuspension respectively for 48 h.RESULTS:Content of ACM in MPNS-ACM was 12.0% andthe average diameter of the particles was 210 nm.Theinhibitory rates of ACM (8 mg/kg bin),high dosage of MPNS-ACM (8 mg/kg bm),low doe,age of MPNS-ACM (1.6 mg/kg bin)and MPNS on human gastric carcinoma in nude mice were22.63%,52.55%,30.66% and 10.22%,respectively.Therewas a significant decrease in the number of CFU-GM ofbone marrow in ACM group compared with control group,whereas no obvious change was observed in that of thenanosphere groups.The values of 50% inhibitionconcentration (IC50) of ACM,MPNS and MPNS-ACM were0.09,97.78 and 1.07μg/mL,respectively. CONCLUSION:The tumor inhibitory rate of MPNS-ACMwas much higher than that of ACM under magnetic fieldand the inhibition on bone marrow was alleviatedsignificantly compared with ACM group.
AIM: To evaluate the effect of aclacinomycin A-loaded magnetic polybutylcyanoacrylate nanoparticles on gastrictumor growth in vivo and in vitro. METHODS: Magnetic polybutylcyanoacrylate (PBCA) nanospheres encapsulated with aclacinomycin A (MPNS-ACM) were prepared by interracial polymerization. Particlesize, shape and drug content were examined.FemaleBABL / c nude mice were implanted with MKN-45 gastriccarcinoma tissues subcutaneously to establish humangastric carcinoma model.The mice were randomly dividedinto 5 groups of 6 each: ACM group (8 mg / kg bm); groupof high dosage of MPNS Group of low dosage of MPNS-ACM (1.6 mg / kg bin); group of magnetic PBCA nanospheres (MPNS) and control group (normal saline). Magnets (2.5 T) were implanted into the tumor masses in all of the mice one day before the therapy. Abbove-mentioned drugs were administered intravenouslyto the mice of every group on the first day and sixth day. How the mice were sacrificed, the tumor weight wasmeasured, and the assay of granu locyte-macrophagecolony forming-unit (CFU-GM) was performed on semi-solid culture. White blood cell, alanine aminotransferase and creatine were examined. 3- [4-dimethylthiazol-2-yl] -2,5-diphenyltetrazoliurn bromide (MTT) was used toexamine the viability of MKN-45 cells after incubation with different concentrations of ACM, MPNS and MPNS-ACMsuspension for 48 h.RESULTS: Content of ACM in MPNS-ACM was 12.0% and the average diameter of the particles was 210 nm.The inhibition high dose of MPNS-ACM (8 mg / kg bm), low doe, age of MPNS-ACM (1.6 mg / kg bin) and MPNS on human gastric carcinoma in nude mice were 22 .63%, 52.55%, 30.66% and 10.22%, respectively. There was a significant decrease in the number of CFU-GM ofbone marrow in ACM group compared with control group, there no obvious change was observed in that of thenanosphere groups.The values of 50% inhibition of concentration (IC50) of ACM, MPNS and MPNS-ACM were 0.09, 97.78 and 1.07 μg / mL, respectively. CONCLUSION: The tumor inhibitory rate of MPNS-ACM was much higher than that of ACM under magnetic field and the inhibition on bone marrow was alleviated slightly beneficially with ACM group.