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目的探讨在类风湿关节炎(RA)中,白三烯B4(LTB4)能否通过促进核因子KappaB受体激活剂配体(RANKL)的表达,起到间接分化破骨细胞的作用。方法利用RA滑膜成纤维细胞(RAFLs)和人外周血单核细胞的共培养体系,对照组2.5ng/ml巨噬细胞集落刺激因子(M-CSF)刺激、实验a组2.5ng/mlM-CSF+10-8mol/LLTB4刺激、实验b组2.5ng/mlM-CSF+10-8mol/LLTB4+100ng/ml骨保护素(OPG)刺激,培养3周后行抗酒石酸酸性磷酸酶(TRAP)细胞化学染色,通过计数多核性TRAP酶染色阳性的破骨细胞样细胞,比较各组的分化破骨细胞作用。结果对照组几乎没有破骨细胞样细胞,而实验a组则出现较多的破骨细胞样细胞,实验b组则与对照组相似,几乎没有破骨细胞样细胞。结论在RA中,LTB4能够通过促进RAFLs细胞RANKL的表达来间接分化破骨细胞。
Objective To investigate whether leukotriene B4 (LTB4) can indirectly differentiate osteoclasts by promoting the expression of nuclear factor KappaB receptor activator ligand (RANKL) in rheumatoid arthritis (RA). Methods The co-culture system of RA synovial fibroblasts (RAFLs) and human peripheral blood mononuclear cells (PBMCs) was stimulated with 2.5ng / ml macrophage colony-stimulating factor (M-CSF) CSF + 10-8mol / LLTB4 stimulation, stimulation with 2.5ng / ml M-CSF + 10-8mol / LLTB4 + 100ng / ml osteoprotegerin (OPG) in experimental group b, and after 3 weeks of culture, the tartrate-resistant acid phosphatase (TRAP) The effect of differentiated osteoclasts in each group was compared by counting multinucleated TRAP-positive osteoclast-like cells. Results There were almost no osteoclast-like cells in the control group, but more osteoclast-like cells in the experimental group a, while the experimental group b was similar to the control group with almost no osteoclast-like cells. Conclusion In RA, LTB4 can differentiate osteoclasts indirectly by promoting the expression of RANKL in RAFLs.