论文部分内容阅读
用凝胶过滤法对水溶性蛋白（ＷＳＰ）分析的结果示皮质点状混浊晶体β＿１－晶体蛋白峰消失；皮质性白内障高分产量（ＨＭ）＋α－晶体蛋白的相对含量显著升高；核性白内障ＨＭ＋α－和β－晶体蛋白升高，γ－晶体蛋白降低。１２烷基磺酸钠－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）示透明晶体尿素溶蛋白（ＵＳＰ）的主要成分为αβ链，各型白内障晶体ＵＳＰ中２８和２３ｋｕ的β－晶体蛋白多肽含量明显升高。透明及各型白内障晶体纤维细胞膜内在蛋白的主要成分为２７主要内在蛋白（ＭＩＰ）和２３ｋｕ的多肽，各型白内障晶体ＭＩＰ的相对含量无明显降低。
The results of gel filtration analysis of water-soluble protein (WSP) showed that the peak of β_1-crystal protein in cortical cataractous opacity disappeared; the relative content of high-yield protein (HM) and α-crystal protein in cortical cataract increased significantly; Cataract HM α- and β-crystallin increased, γ-crystallin decreased. 12 sodium alkyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the main component of the transparent crystal urea hemolysin (USP) is the αβ chain, the content of β-crystal protein of 28 and 23 ku in the various types of cataract crystals USP Significantly increased. The main components of the intrinsic protein of the transparent and various types of cataract crystal fiber cell membrane were 27 major intrinsic protein (MIP) and 23ku polypeptide, and the relative content of MIP in all types of cataract crystals was not significantly reduced.