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目的探讨七叶皂甙钠对大鼠大脑中动脉闭塞缺血再灌注后缺血区神经元凋亡的影响。方法选用健康雄性Wistar大鼠140只,用线栓法建立大鼠大脑中动脉缺血再灌注模型,切取不同时间段缺血半暗带和中心区皮质组织,采用逆转录聚合酶链反应(RTPCR)测定凋亡相关基因(Fas、FasL与Bcl2)转录水平的变化;干预组再灌注即刻开始腹腔内注射七叶皂甙钠(5mg/kg),以后5mg·kg1·24h1治疗量,而对照组再灌注即刻注射等量生理盐水;对选取的脑组织块行原位末端转移酶标记(TUNEL),计数缺血半暗带和中心区凋亡细胞数目。结果干预组各时段大鼠脑缺血组织神经元凋亡计数明显低于对照组(P<0.01),表达高峰下降明显,干预组脑缺血组织中Fas、FasLmRNA表达明显下调,同时可见Bcl2mRNA表达上调。结论七叶皂甙钠具有抗神经元凋亡作用;调节神经元凋亡相关基因表达水平可能是其机制之一。
Objective To investigate the effect of aescinate sodium on apoptosis of ischemic neurons after middle cerebral artery occlusion in rats. Methods A total of 140 healthy male Wistar rats were selected. The model of middle cerebral artery ischemia and reperfusion in rats was established by thread embolism method. Cortical tissue in the ischemic penumbra and the central area at different time points was cut and subjected to reverse transcription polymerase chain reaction (RTPCR). The changes in the transcription levels of apoptosis-related genes (Fas, FasL, and Bcl2) were measured; in the intervention group, saponin sodium (5 mg/kg) was intraperitoneally injected immediately after the reperfusion, and the subsequent 5 mg·kg1·24 h1 treatment amount was observed in the control group. Immediate saline injection was performed by perfusion. The selected brain tissue was subjected to in situ terminal transferase labeling (TUNEL) to count the number of apoptotic cells in the ischemic penumbra and the central region. Results The number of apoptotic neurons in the cerebral ischemic tissue of rats in the intervention group was significantly lower than that in the control group (P<0.01). The peak expression decreased significantly in the intervention group. The expression of Fas and FasL mRNA was significantly down-regulated in the intervention group and the expression of Bcl2 mRNA was also observed. Increased. Conclusion Aescinate has an anti-apoptotic effect on neurons; regulation of neuronal apoptosis-related gene expression may be one of the mechanisms.