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以辣椒(Capsicum annuum L)品种“哈椒一号”为试材,选用无菌苗子叶、下胚轴和带柄子叶为外植体,采用正交设计探讨不同外植体、激素组合和AgNO_2浓度等因素对不定芽分化的影响,筛选出了三种外植体不定芽诱导的最适培养基。试验结果表明:在不定芽的分化中,6-BA与IAA相配合使叶片不定芽分化频率明显提高,BA/IAA的比例对不定芽分化有明显影响。并且6-BA 6 mg/L+IAA 2 mg/L的组合能够达到最高的诱导率。AgNO_3不但显著提高了不定芽诱导的频率,并且还缩短了不定芽的发生时间。另外,三种外植体的不定芽诱导率,带柄子叶最高,其次是子叶,下胚轴最差。最适不定芽培养基:MS+6-BA 6 mg/L+IAA 2 mg/L+AgNO_2 4 mg/L,pH 5.8,最高不定芽分化率达88.2%。
With pepper (Capsicum annuum L.) variety “Pepper No.1” as test material, sterile seedling leaves, hypocotyls and cotyledons were used as explants. Orthogonal design was used to investigate the effects of different explants, hormone combinations And AgNO 2 concentration on the differentiation of adventitious buds were studied. Three optimal explants of adventitious buds were selected. The results showed that in the adventitious bud differentiation, the differentiation frequency of adventitious buds increased obviously with the combination of 6-BA and IAA, and the ratio of BA / IAA significantly affected the differentiation of adventitious buds. And the combination of 6-BA 6 mg / L + IAA 2 mg / L achieved the highest induction rate. AgNO_3 not only significantly increased the frequency of adventitious bud induction, but also shortened the time of adventitious buds. In addition, the induction rate of adventitious buds of the three explants was the highest with cotyledons, followed by the cotyledons, and the lowest among hypocotyls. The optimal adventitious buds medium was MS + 6-BA 6 mg / L + IAA 2 mg / L + AgNO 4 4 mg / L, pH 5.8, and the highest adventitious bud differentiation rate was 88.2%.