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昆虫体内的解毒酶活性是反映其抗药性水平的主要生理指标,本文比较了桔小实蝇Bactrocera dorsalis的几种解毒酶活性和抗药性水平关系。测定了9个地理品系和相对敏感品系的桔小实蝇成虫的三种解毒酶,即多功能氧化酶(Mixed function oxidase,MFO)、羧酸酯酶(Carboxylesterase,CarE)和谷胱甘肽S-转移酶(Glutathione S-transferase,GST)的活性,利用药膜法测定其对敌百虫(Trichlorphon)、高效氯氰菊酯(β-cypermethrin)和阿维菌素(Avermectin)的抗性水平,比较了各地理品系的桔小实蝇这些酶活性和对不同杀虫剂的抗性水平的关系,并作通径分析。结果表明:广东广州地区桔小实蝇体内的MFO-O-脱甲基活性最高,为相对敏感品系酶活性的1.4782倍;而广东茂名地区品系酶活性最低,只有0.8649倍。广东惠州地区桔小实蝇体内CarE活性最高,为相对敏感品系酶活性1.8147倍;而广西南宁地区的桔小实蝇体内酶活性最低,为敏感品系的0.9636倍。广东茂名地区桔小实蝇GST活性最高,是相对敏感品系2.2557倍;而广东广州地区桔小实蝇GST活性最低,只有相对敏感品系的1.1622倍。抗性水平表明:各地理品系对敌百虫的抗性水平为相对敏感品系的2.1552倍至100.2271倍之间,对高效氯氰菊酯的抗性水平在1.0065到26.0026倍之间,对阿维菌素的抗性水平在2.3353倍至29.0688倍之间。相关性分析表明:桔小实蝇体内的GST活性和对敌百虫抗性水平的相关系数为0.41,存在显著性正相关;CarE活性与桔小实蝇对高效氯氰菊酯抗性水平存在极显著正相关,相关系数为0.50。通径分析结果表明:GST活性对敌百虫的抗药性水平的直接通径系数为0.4414,对敌百虫的抗性上升起到正向作用;MFO-O-脱甲基活性、CarE活性和GST活性对高效氯氰菊酯抗性水平的直接通径系数分别为0.3311,0.4946和0.1775,均起到正向作用;GST活性与阿维菌素的抗性水平的直接通径系数很小,为0.0668。结果显示了桔小实蝇的解毒酶与抗药性水平关系密切,在抗性发展中起到了促进作用。
Insect detoxification enzyme activity is the main physiological index to reflect the level of drug resistance. In this paper, the relationship between several detoxification enzyme activity and drug resistance of Bactrocera dorsalis was compared. Three detoxification enzymes, namely Mixed Function Oxidase (MFO), Carboxylesterase (CarE) and Glutathione S - Glutathione S-transferase (GST) activity was measured. The resistance levels to Trichlorphon, β-cypermethrin and Avermectin were determined by membrane assay The physiological strains of the orange fruit fly these enzyme activity and the different levels of insecticide resistance, and for path analysis. The results showed that the MFO-O-demethylation activity was the highest in Guangzhou, Guangdong Province, 1.4782 times of that of the sensitive strains. The activity of the enzyme in Maoming, Guangdong Province was the lowest, only 0.8649 times. The carE activity in the fruit flies in Huizhou, Guangdong province was the highest, which was 1.8147 times higher than that in the relatively sensitive strains. However, the enzyme activity in the fruit flesh was lowest in Nanning, Guangxi, which was 0.9636 times of the susceptible strain. The GST activity was the highest in Maoming, Guangdong Province, which was 2.2557 times that of the susceptible strain, while the GST activity was the lowest in Guangzhou, Guangdong Province, only 1.1622 times that of the sensitive strain. The results showed that the resistant levels of each geographical line to trichlorfon were between 2.1552 times and 100.2271 times that of the susceptible lines, between 1.0065 and 26.0026 times of those of cypermethrin, Resistance levels ranged from 2.3353 times to 29.0688 times. Correlation analysis showed that there was a significant positive correlation between GST activity and trichlorfon resistance level in Trichosanthes; there was a significant positive correlation between CarE activity and beta-cypermethrin resistance Relevant, the correlation coefficient is 0.50. The results of path analysis showed that the direct path coefficient of GST activity on the resistance of trichlorfon was 0.4414, which had a positive effect on the increase of trichlorfon resistance. The activity of MFO-O-demethylation, CarE activity and The direct path coefficients of GST activity to beta-cypermethrin resistance were 0.3311, 0.94946 and 0.1775, respectively. Both of them played a positive role. The direct path coefficient of GST activity and abamectin resistance was very small at 0.0668. The results showed that the detoxification enzyme of the fruit fly was closely related to the drug resistance, and played a promoting role in the development of the resistance.