论文部分内容阅读
观察实验性大鼠脑挫伤后NSE、S－100的时间性变化规律，为法医学推断脑挫伤形成时间提供新的手段。采用改进的Feeney氏落体打击致Wistar大鼠脑挫伤模型，进行免疫组织化学SP法染色，并利用图像分析仪对免疫组化染色阳性细胞灰度和面积进行测量，SAS统计软件分析，结果显示：NSE阳性神经元灰度与面积在伤后1h至2d呈下降趋势，至伤后12h阳性细胞灰度、面积降到最小值（P＜0．01）；S－100阳性细胞面积和灰度伤后呈上升趋势，至伤后4dS－100阳性细胞灰度、面积达到最大值，与对照组及其它实验组比较均有显著性差异（P＜0．01），伤后5d仍保持较高水平；NSE、S－100免疫组织化学染色阳性细胞的数量、灰度、面积改变有时间规律。推断2d内脑挫伤可用NSE作为主要指标，推断2～5d的脑挫伤则以S－100改变为主。
Observe the temporal change of NSE and S-100 after experimental contusion in rats, and provide a new method for forensic science to infer the time of cerebral contusion. The model of brain contusion in Wistar rats was induced by modified Feeney ’s drop body and immunohistochemical SP method was used for staining. Immunohistochemistry was used to measure the gray level and area of immunohistochemical positive cells by image analyzer. The results of SAS statistical software showed that: The gray scale and area of NSE-positive neurons decreased from 1 h to 2 d post-injury, and reached the minimum (P <0.01) at 12 h after injury. The area of S-100 positive cells and grayscale injury (P <0.01). The level of S-100-positive cells reached the maximum at 4 days after injury, which was significantly higher than that of the control group and other experimental groups (P <0.01) ; NSE, S-100 immunohistochemical staining of the number of positive cells, grayscale, change the area of time with the law. Inferred 2d intracerebral contusion NSE can be used as a major indicator, infer 2 ~ 5d brain contusion is the main S-100 changes.