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目的:研究新生大鼠高氧暴露后不同时期肺组织巨噬细胞亚型的表达情况。方法:将64只新生SD大鼠随机分为对照组和高氧组,每组32只。高氧组采用体积分数为95%的氧暴露7 d,对照组仅置于同一屋子空气环境中(氧体积分数为21%)。采用细胞流式术对暴露后的不同时期(第1、3、7、21天)大鼠支气管肺泡灌洗液(BLAF)内3种亚类的巨噬细胞(经典活化巨噬细胞、调节巨噬细胞和损伤修复巨噬细胞)进行双阳性筛选;光镜观察并盲法评分比较肺组织病理学评分。结果:与对照组相比,高氧组新生鼠体质量明显较低(P<0.05);高氧组各个时间点F4/80+巨噬细胞含量均增高(P<0.05),IL-12表达增加(P<0.05);高氧组于第1、7、21天CD163表达增加(P<0.05),而第3天两组CD163表达差异无统计学意义(P>0.05);高氧组于第1、3、7天IL-10表达增加(P<0.05),第21天两组IL-10表达差异无统计学意义(P>0.05)。结论:高氧暴露新生大鼠肺组织存在巨噬细胞亚型表达差异,与肺组织的损伤具有一定的相关性。
Objective: To study the expression of macrophage subtypes in lung tissue of neonatal rats exposed to hyperoxia. Methods: 64 newborn SD rats were randomly divided into control group and hyperoxia group, 32 in each group. The rats in the hyperoxia group were exposed to 95% oxygen for 7 days. The control group was only exposed to the same room atmosphere (oxygen volume fraction was 21%). Three types of macrophages in the bronchoalveolar lavage fluid (BLAF) (classic activated macrophages, Phagocytosis and injury repair macrophages) double positive screening; light microscopy and blind method score lung histopathological score. Results: Compared with control group, the body weight of neonatal rats in hyperoxia group was significantly lower (P <0.05); the content of F4 / 80 + macrophages in hyperoxia group was higher (P <0.05) and the expression of IL-12 (P <0.05). The expression of CD163 on day 1, day 2 and day 2 was increased in hyperoxia group (P <0.05), while the expression of CD163 on day 3 was not significantly different (P> 0.05) The expression of IL-10 increased on the 1st, 3rd, 7th day (P <0.05), and there was no significant difference on the 21st day between the two groups (P> 0.05). CONCLUSION: The expression of macrophage subtypes in the lungs of neonatal rats exposed to hyperoxia exposure has a certain correlation with the damage of lung tissue.