钌(Ⅱ)-联吡啶-丙酮酸-Ce(Ⅳ)化学发光体系测定人血清中丙酮酸的研究

• 作 者: 凌连生 何治柯 蔡汝秀
• 机 构: 武汉大学化学系!武汉，430072,武汉大学化学系!武汉，430072,武汉大学化学系!武汉，430072
• 出 处: 高等学校化学学报
• 发表日期: 1997年12期
• 关键词: 化学发光体系 Ce 联吡啶 丙酮酸 人血清 电致化学发光 发光行为 平行测定 检测限 储备液

The reaction of Ce (Ⅳ) with Ru (bipy) (bipy=2, 2-bipyridine) and pyruvate produces chemiluminescence (CL) in aqueous solution. The effect of concentration of suIfate acid, Ce(Ⅳ) and Ru(bipy) on the CL intensity was studied. The results showed that CL intensity was linearly related to the concentration of pyruvate over the range of 4. 0 ×10-8~8. 0×10-6 mol/L under the optimum conditions. The method was applied successfully to determine pyruvate in normal human serum.

The reaction of Ce (IV) with Ru (bipy) (bipy = 2, 2-bipyridine) and pyruvate (CL) in aqueous solution. The effect of concentration of sufate acid, Ce the CL intensity was studied. The results showed that CL intensity was linearly related to the concentration of pyruvate over the range of 4. 0 × 10 -8 ~ 8. 0 × 10 -6 mol / L under the optimum conditions. The method was applied successfully to determine pyruvate in normal human serum.