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目的检测食管鳞癌组织中F-box蛋白4(FBX4)和端粒重复序列结合因子1相互作用核蛋白2(TIN2) mRNA表达,并分析其与食管鳞癌相关临床指标的关系。方法收集110例明确诊断的食管鳞癌中心组织及其30例癌旁组织,采用SBYR Green实时荧光定量PCR方法检测FBX4和TIN2基因在食管鳞癌中心组织和癌旁组织mRNA水平的表达,并分析FBX4和TIN2 mRNA表达临床相关指标的关系。结果 FBX4 mRNA在食管鳞癌组织中的相对表达量为0.24±0.43(n=110),而在肿瘤旁组织中为0.40±0.27(n=30),两者差异具有统计学意义(P=0.008);食管鳞癌组织中TIN2 mRNA的相对表达量为1.59±1.19(n=101);肿瘤旁为2.54±1.89(n=30),两者差异具有统计学意义(P=0.000)。TIN2 mRNA表达在临床各参数不同分组内均未发现有统计学差异(P>0.05),而FBX4 mRNA表达虽在不同性别、年龄、钡餐分型、临床分期和肿瘤长度中均没有统计学差异(P>0.05),但在不同病理分型中存在统计学差异,即相对于高分化鳞癌,低分化和中分化的鳞癌组织中的FBX4 mRNA表达增加,差异具有统计学意义(P<0.05)。FBX4和TIN2在肿瘤组织中的表达呈正相关(r=0.671,P=0.000)。结论 FBX4和TIN2 mRNA在食管鳞癌组织中均表现表达增高,且存在直线相关关系,提示FBX4和TIN2可能在食管癌的发生发展中具有重要的临床意义;肿瘤分化程度与FBX4表达相关,而与TIN2不相关,表明肿瘤分化还与FBX4介导的泛素化相关。
Objective To detect the expression of F-box protein 4 (FBX4) and telomeric repeat binding protein-1 (TIN2) mRNA in esophageal squamous cell carcinoma (ESCC) tissues and to analyze their relationship with clinical parameters of esophageal squamous cell carcinoma. Methods Totally 110 cases of esophageal squamous cell carcinoma and 30 cases of paracancerous tissues were collected. SBYR Green real-time PCR was used to detect the mRNA expression of FBX4 and TIN2 in esophageal squamous cell carcinoma tissues and adjacent tissues Relationship between FBX4 and TIN2 mRNA expression in clinically relevant indicators. Results The relative expression of FBX4 mRNA in esophageal squamous cell carcinoma was 0.24 ± 0.43 (n = 110) and 0.40 ± 0.27 (n = 30) in paracancerous tissues, the difference was statistically significant (P = 0.008 ). The relative expression of TIN2 mRNA in esophageal squamous cell carcinoma tissues was 1.59 ± 1.19 (n = 101). The tumor size was 2.54 ± 1.89 (n = 30), the difference was statistically significant (P = 0.000). There was no significant difference in the expression of TIN2 mRNA in different clinical groups (P> 0.05), while FBX4 mRNA expression was not statistically different in different sex, age, barium meal type, clinical stage and tumor length P> 0.05). However, there was a significant difference in different pathological types, that is, the expression of FBX4 mRNA in poorly differentiated and moderately differentiated squamous cell carcinoma was significantly higher than that in well differentiated squamous cell carcinoma (P <0.05) ). The expression of FBX4 and TIN2 in tumor tissue was positively correlated (r = 0.671, P = 0.000). Conclusions The expression of FBX4 and TIN2 mRNA in esophageal squamous cell carcinoma both increased and there was a linear correlation between FBX4 and TIN2 mRNA, suggesting that FBX4 and TIN2 may have important clinical significance in the occurrence and development of esophageal cancer. The degree of differentiation is related to the expression of FBX4, TIN2 was not correlated, indicating that tumor differentiation is also associated with FBX4-mediated ubiquitination.