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目的建立柴黄片制剂中柴胡皂苷b1、b2的HPLC含量测定方法。方法采用Hypersil C18色谱柱(4.6mm×250 mm,5μm);流动相:甲醇-水(75∶25);流速:1.0 mL·min-1;检测波长:254nm;柱温:30℃。结果柴胡皂苷b1的线性范围为0.015～0.120μg(r=0.999 5),柴胡皂苷b2的线性范围为0.057～0.456μg(r=0.999 8),柴胡皂苷b1、b2的平均加样回收率分别为97.2%、96.9%,RSD分别为2.0%、1.6%。结论本方法简便、可靠、重现性好,可用于柴黄片中柴胡皂苷b1、b2的含量测定,为完善含柴胡类成方制剂的质量评价体系提供参考。
Objective To establish a HPLC method for the determination of saikosaponin b1 and b2 in Shixian tablet. Methods The mobile phase consisted of Hypersil C18 column (4.6 mm × 250 mm, 5 μm), mobile phase of methanol-water (75:25), flow rate of 1.0 mL · min-1, detection wavelength of 254 nm and column temperature of 30 ℃. Results The linear range of saikosaponin b1 was 0.015 ~ 0.120μg (r = 0.999 5). The linear range of saikosaponin b2 was 0.057 ~ 0.456μg (r = 0.999 8) The rates were 97.2% and 96.9%, respectively, with RSDs of 2.0% and 1.6%, respectively. Conclusion The method is simple, reliable and reproducible. It can be used to determine the content of saikosaponin b1 and b2 in Qixian tablets, and provide a reference for improving the quality evaluation system of Bupleurum Preparations.