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目的:探讨碱性成纤维细胞生长因子(bFGF)、骨形态发生蛋白(BMP)-12基因序贯转染兔脂肪干细胞向成纤维细胞分化的可能性。方法:2017年1月至2018年12月，原代培养兔脂肪干细胞(取自新西兰大耳兔颈背部皮下脂肪)，细胞表面抗原CD44、CD49d、CD106检测结合成骨细胞诱导分化对培养的细胞进行鉴定，脂质体介导的方法序贯转染BMP-12和bFGF基因，蛋白质印迹法(Western blot)检测目的基因BMP-12和bFGF蛋白的表达；实时荧光定量聚合酶链反应(Real-time PCR)检测转染细胞Ⅰ型胶原和弹性蛋白RNA的表达情况。符合正态分布的计量资料以均数±标准差(n ±n s)表示，应用单因素方差分析比较组间差异。n 结果:兔脂肪组织中分离出来的脂肪间充质干细胞(ADSCs) CD44、CD49d表达阳性，CD106表达呈阴性。Western blot检测筛选后的ADSCs稳定表达BMP-12(0.229±0.005)和bFGF(0.208±0.019)蛋白(n F＝120.221，n P<0.05)，差异有统计学意义。双基因转染的ADSCsⅠ型胶原(2.250±0.007，n F＝340.103，n P<0.05)和弹性蛋白(1.807±0.008，n F＝107.314，n P<0.05)的mRNA表达最高，差异均有统计学意义。n 结论:pcDNA3.1＋绿色荧光蛋白(GFP)-BMP-12和pcDNA3.1＋红色荧光蛋白(RFP)-bFGF可以促进ADSCs向成纤维细胞分化，可能在组织工程技术修复韧带损伤中具有重要作用。“,”Objective:To investigate the possibility of basic fibroblast growth factor (bFGF) and bone morphogenetic protein (BMP)-12 genes into rabbit adipose stem cells to differentiate into fibroblasts.Methods:The study was conducted from January 2017 to December 2018. The cells were derived from subcutaneous fat in the neck and back of the New Zealand big-eared rabbits. Rabbit adipose-derived stem cells (ADSCs) were cultured, and CD44, CD49d and CD106 were used to identify the cultured cells. Liposome was used to sequentially transfect BMP-12 and bFGF genes. Western blotting was used to detect the protein expression of BMP-12 and bFGF. Real time-PCR assay was used to detect the mRNA expression of type Ⅰ collagen and elastin. One-way ANOVA was used to compare the differences among the groups. n P<0.05 was considered statistically significant.n Results:The ADSCs isolated from rabbit adipose tissue were positive for CD44 and CD49d while negative for CD106. The expression of BMP-12 (0.229±0.005) and bFGF (0.208±0.019) proteins was stable in the selected ADSCs (n F=120.221, n P<0.05). The mRNA expression of type Ⅰ collagen (2.250±0.007,n F=340.103, n P<0.05) and elastin (1.807±0.008,n F=107.314, n P<0.05) in ADSCs transfected with two genes was the highest and the difference was statistically significant.n Conclusion:PcDNA3.1+ green fluorescent protein (GFP)-BMP-12 and pcDNA3.1+ red fluorescence protein (RFP)-bFGF could promote the differentiation of ADSCs into fibroblasts, which may play an important role in tissue engineering to repair ligament damage.