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关于烟酰胺辅酶催化还原的机理,即所谓H~-的一步转移和单电子转移引发的多步转移(其中又分和两种形式),在生物有机化学中一直存有争议。对以六氰合铁(Ⅲ)酸钾作为电子受体与NAD(P)H辅酶模型(如1-苄基-1,4-二氢烟酰胺(BNAH)及其类似物)的反应(1式)来说,在文献中[4,5]中的研究表明该反应由(i)BNAH向K_3Fe(CN)_6转移电子的慢过程和(ii)BNAH~+向体系中存在的碱(B:)转移质子的快过程两部分组成,其假一级动力学速率(k_(obs)的倒数1/k_(obs))与[K_4Fe(CN)_6]呈线性。
The mechanisms involved in the catalytic reduction of nicotinamide coenzymes, the so-called one-step transfer of H ~ - and the single-electron transfer-initiated multistep transfer, both sub-divided and two forms, have been controversial in bioorganic chemistry. The reaction of potassium hexacyanoferrate (III) as an electron acceptor with a NAD (P) H coenzyme model such as 1-benzyl-1,4-dihydronicotinamide (BNAH) and the like Studies in the literature [4,5] show that this reaction consists of a slow process of (i) the transfer of electrons from BNAH to K_3Fe (CN) _6 and (ii) a slow release of BNAH ~ The proton-first-order kinetic rate (reciprocal 1 / k obs of k obs) is linear with [K_4Fe (CN) 6).