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目的:观察白细胞介素17(Interleukin-17,IL-17)在乙肝孕妇和正常孕妇胎盘中表达差异来反映胎盘内的细胞免疫应答的情况,为外源性细胞因子在增强机体免疫、清除病毒及阻断和治疗HBV宫内感染提供新的科学依据。方法:选择择期剖宫产的足月妊娠妇女46例,其中血清HBs Ag(+)30例,血清HBs Ag(-)的对照组16例,采用免疫组化方法检测胎盘组织IL-17的表达。结果:IL-17主要表达在胎盘绒毛的合体滋养层细胞胞浆、毛细血管内皮细胞胞浆以及基质中。血清HBs Ab(+)孕妇与HBs Ab(-)孕妇比较,无显著差异(P>0.05),HBs Ag(+)乙肝孕妇与对照组的表达比较差异有统计学意义(P<0.05),HBe Ag(+)孕妇与HBe Ag(-)孕妇的表达比较差异有统计学意义(P<0.05)。结论 :IL-17在乙肝孕妇胎盘组织中的表达高于正常孕妇,IL-17的表达不但加强了机体的防御功能,且能促进炎性反应。
Objective: To observe the expression of Interleukin-17 (IL-17) in the placenta of pregnant women and normal pregnant women with hepatitis B to reflect the cellular immune response in the placenta, to enhance the immunity of exogenous cytokines and eliminate the virus And blocking and treatment of HBV intrauterine infection provide a new scientific basis. Methods: A total of 46 full-term pregnant women undergoing cesarean section were enrolled. Among them, 30 cases of serum HBsAg (+) and 16 cases of serum HBsAg (-) control group were selected. The expression of IL-17 in placenta was detected by immunohistochemistry . Results: IL-17 was mainly expressed in the syncytiotrophoblast cytoplasm, capillary endothelial cell cytoplasm and matrix of placental villi. Serum HBs Ab (+) pregnant women and HBs Ab (-) pregnant women compared with no significant difference (P> 0.05), HBsAg (+) hepatitis B pregnant women and the control group, the difference was statistically significant (P < The expression of Ag (+) pregnant women and HBe Ag (-) pregnant women was significantly different (P <0.05). Conclusion: The expression of IL-17 in placenta of pregnant women with hepatitis B is higher than that of normal pregnant women. The expression of IL-17 not only strengthens the body’s defensive function but also promotes the inflammatory reaction.